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Properties and in vitro Translation of Maize Dwarf Mosaic Virus RNA

P. H. Berger^1,

¹ Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546
² Department of Biology, Indiana University of Pennsylvania, Indiana, Pennsylvania 15705
³ Department of Plant Pathology, Iowa State University, Ames, Iowa 50011
and⁴ Department of Plant Pathology, University of Minnesota, St Paul, Minnesota 55108, U.S.A.

The RNA of maize dwarf mosaic virus strain A (MDMV-A) was characterized and compared with that of strain B (MDMV-B). Glyoxal-treated MDMV-A RNA has an M_r of 3.32 x 10⁶ measured in agarose gels, compared with that of MDMV-B which is 3.41 x 10⁶ under the same conditions. MDMV-A RNA has a T_m of 50.7 °C and a hyperchromicity of 23.3%, which are higher than those reported for MDMV-B RNA. Translation products of both RNAs in a rabbit reticulocyte cell-free system ranged from 23K to 121K. Four major polypeptide products of 57K, 91K, 100K and 117K were produced by MDMV-A RNA-directed translation, and four major products of 39K, 55K, 86K and 121K by MDMV-B RNA. Protein profiles from the two RNAs were distinct including translation products that comigrated with authentic coat protein at 37K (MDMV-A) or 39K (MDMV-B) for each strain. Anti-virion sera immunoprecipitated polypeptide products from each strain. Distinct subsets of translation products were immunoprecipitated with antisera to tobacco etch virus 49K and 54K nuclear inclusion proteins. No cell-free translation products were recognized by antisera to potato virus Y (PVY) or tobacco vein mottling virus (TVMV) helper components or antisera to the cylindrical inclusion proteins of PVY or TVMV.

Keywords: MDMV, RNA, translation in vitro

Present address: Department of Plant, Soil, and Entomological Sciences, University of Idaho, Moscow, Idaho 83832, U.S.A.

Received 2 November 1988; accepted 17 February 1989.