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J Gen Virol 70 (1989), 1913-1919; DOI 10.1099/0022-1317-70-7-1913
© 1989 Society for General Microbiology
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Structure and Expression of an Integrated Human Papillomavirus Type 16 Genome Amplified in a Cervical Carcinoma Cell Line

Hiroshi Shirasawa1, Yoshimi Tomita1, Akira Fuse1, Tomoko Yamamoto1, Hideki Tanzawa1, Souei Sekiya2, Hiroyoshi Takamizawa2 and Bunsiti Simizu1

1 Department of Microbiology
and2 Department of Obstetrics and Gynecology, School of Medicine, Chiba University, Chiba 280, Japan

A cellular sequence containing the integrated human papillomavirus type 16 genome in a cervical carcinoma cell line QG-U was cloned and analysed. The transcriptionally active viral genome disrupted at the E2 and L2 open reading frames was amplified with its flanking sequences.

Keywords: HPV-16 genome, cervical carcinoma cell line, flanking sequences

Received 5 December 1988; accepted 17 February 1989.




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