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Examination of Eight Cases of Multiple Sclerosis and 56 Neurological and Non-neurological Controls for Genomic Sequences of Measles Virus, Canine Distemper Virus, Simian Virus 5 and Rubella Virus

The Queen's University of Belfast
¹ Multiple Sclerosis Laboratory, Institute of Pathology, Royal Victoria Hospital, Belfast BT12 6BL
and² NICGENE, School of Biology and Biochemistry, 97 Lisburn Road, Belfast BT9 7BL, U.K.

In situ hybridization studies have been carried out on brain samples from eight cases of multiple sclerosis (MS) and 56 non-neurological and neurological controls, using single-stranded ³⁵S-labelled RNA probes prepared against genomic RNA sequences of measles virus, canine distemper virus, rubella virus and simian virus 5. Foci of hybridization were found using probes against the measles virus nucleocapsid protein (N), phosphoprotein and fusion protein gene sequences in two of the MS cases, and also in one control, a case of disseminated cytomegalovirus infection with spinal cord necrosis. This result was confirmed using biotinylated probes prepared against the measles virus N genomic sequence. No hybridization was found in any of the MS or control cases using any of the other viral genome-specific probes.

Keywords: measles virus RNA sequences, multiple sclerosis, in situ hybridization

Received 20 December 1988; accepted 10 April 1989.

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