| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Yale Arbovirus Research Unit, Yale University School of Medicine, 60 College Street, New Haven, Connecticut 06510
2 Department of Viral Biology, United States Army Medical Research Institute for Infectious Diseases, Fort Detrick, Frederick, Maryland 21701-5011
3 Department of Molecular Biology
and4 Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, D.C. 20307
and5 Department of Biochemistry and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts 01003, U.S.A.
Expression of antigenic fragments of the Japanese encephalitis virus envelope protein (E) in Escherichia coli has been used to define the boundaries of an antigenic domain that contains the binding sites for 10 anti-E monoclonal antibodies (MAbs). All of these antibodies neutralized the virus in vitro and some of them passively protected mice from a fatal virus challenge. We have shown previously that nine of these antibodies react with the antigenic determinants encoded by a 405 bp fragment of viral cDNA. To determine the amino acid sequences of specific determinants, truncated polypeptides were expressed as fusion proteins in E. coli following progressive Bal 31 exonuclease digestion of the 5' and 3' ends of the cDNA fragment. Examination of the immunoreactivity of these polypeptides revealed that the region from methionine 303 to tryptophan 396 was the shortest sequence capable of reacting with any of the 10 MAbs or with a polyclonal, antiviral hyperimmune mouse ascitic fluid. Biochemical tests showed that an intramolecular disulphide cross-linkage between cysteine 304 and cysteine 335 of the E protein sequence was required for presentation of the binding site(s) for these MAbs. Although this 95 amino acid antigenic domain appeared to be capable of forming several conformational neutralizing epitopes, it was not an effective immunogen for inducing neutralizing or protective antibodies in mice.
Keywords: JE virus, envelope protein, glycoprotein, antigenic site
Received 23 December 1988;
accepted 14 April 1989.
This article has been cited by other articles:
|
|
 |
|
  B. Shrestha and M. S. Diamond Role of CD8+ T Cells in Control of West Nile Virus Infection J. Virol., August 1, 2004; 78(15): 8312 - 8321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Diamond, E. M. Sitati, L. D. Friend, S. Higgs, B. Shrestha, and M. Engle A Critical Role for Induced IgM in the Protection against West Nile Virus Infection J. Exp. Med., December 15, 2003; 198(12): 1853 - 1862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kojima, A. Yasuda, H. Asanuma, T. Ishikawa, A. Takamizawa, K. Yasui, and T. Kurata Stable High-Producer Cell Clone Expressing Virus-Like Particles of the Japanese Encephalitis Virus E Protein for a Second-Generation Subunit Vaccine J. Virol., August 15, 2003; 77(16): 8745 - 8755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Saini and S. Vrati A Japanese Encephalitis Virus Peptide Present on Johnson Grass Mosaic Virus-Like Particles Induces Virus-Neutralizing Antibodies and Protects Mice against Lethal Challenge J. Virol., March 15, 2003; 77(6): 3487 - 3494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-W. Lin and S.-C. Wu A Functional Epitope Determinant on Domain III of the Japanese Encephalitis Virus Envelope Protein Interacted with Neutralizing-Antibody Combining Sites J. Virol., February 15, 2003; 77(4): 2600 - 2606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Pan, H.-W. Chen, H.-W. Huang, and M.-H. Tao Protective Mechanisms Induced by a Japanese Encephalitis Virus DNA Vaccine: Requirement for Antibody but Not CD8+ Cytotoxic T-Cell Responses J. Virol., December 1, 2001; 75(23): 11457 - 11463. [Abstract] [Full Text]
|
|
|
|
|
|
E. Konishi, A. Fujii, and P. W. Mason Generation and Characterization of a Mammalian Cell Line Continuously Expressing Japanese Encephalitis Virus Subviral Particles J. Virol., March 1, 2001; 75(5): 2204 - 2212. [Abstract] [Full Text]
|
|
|
|
|
|
G.-J. J. Chang, A. R. Hunt, and B. Davis A Single Intramuscular Injection of Recombinant Plasmid DNA Induces Protective Immunity and Prevents Japanese Encephalitis in Mice J. Virol., May 1, 2000; 74(9): 4244 - 4252. [Abstract] [Full Text]
|
|
|
|
|
|
H.-W. Chen, C.-H. Pan, M.-Y. Liau, R. Jou, C.-J. Tsai, H.-J. Wu, Y.-L. Lin, and M.-H. Tao Screening of Protective Antigens of Japanese Encephalitis Virus by DNA Immunization: a Comparative Study with Conventional Viral Vaccines J. Virol., December 1, 1999; 73(12): 10137 - 10145. [Abstract] [Full Text]
|
|
|
|
|
|
E. Konishi, M. Yamaoka, Khin-Sane-Win, I. Kurane, K. Takada, and P. W. Mason The Anamnestic Neutralizing Antibody Response Is Critical for Protection of Mice from Challenge following Vaccination with a Plasmid Encoding the Japanese Encephalitis Virus Premembrane and Envelope Genes J. Virol., July 1, 1999; 73(7): 5527 - 5534. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. J. Gubler Dengue and Dengue Hemorrhagic Fever Clin. Microbiol. Rev., July 1, 1998; 11(3): 480 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Konishi, M. Yamaoka, Khin-Sane-Win, I. Kurane, and P. W. Mason Induction of Protective Immunity against Japanese Encephalitis in Mice by Immunization with a Plasmid Encoding Japanese Encephalitis Virus Premembrane and Envelope Genes J. Virol., June 1, 1998; 72(6): 4925 - 4930. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
