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J Gen Virol 70 (1989), 2121-2137; DOI 10.1099/0022-1317-70-8-2121
© 1989 Society for General Microbiology
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A Herpes Simplex Virus Type 2 Variant in which a Deletion across the L-S Junction Is Replaced by Single or Multiple Reiterations of Extraneous DNA

June Harland and S. Moira Brown

MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K.

We have isolated and characterized a novel variant of herpes simplex virus type 2 (HSV-2) strain HG52 which has a deletion of 13.5 kb across the L-S junction of the genome, resulting in all of the IRL region, half of the IRS region and the intervening L-S junction ‘a’ sequence(s) being removed. The deleted DNA has been replaced by single or multiple (up to 14) reiterations of a DNA sequence approximately 1 kb in length. Individual genomes within the population range in size from approximately 12 kb smaller than unit length to unit length or marginally larger. The L component of the genome is fixed in the prototype orientation while the S component inverts inefficiently. The variant is viable in tissue culture, is not temperature-sensitive, demonstrates impaired single-cycle growth characteristics and, apart from altered mobility of a single species (29.5K), its polypeptide profile in infected cells appears normal. Southern blot analysis has failed to identify the inserted sequences as being derived from HSV-2.

Keywords: HSV-2, deletion variant, insertion

Received 24 February 1989; accepted 14 April 1989.




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Copyright © 1989 by the Society for General Microbiology.