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Monoclonal Antibodies to Feline Calicivirus

E. G. Routledge

Department of Virology, The University of Newcastle upon Tyne, New Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH, U.K.

We have prepared monoclonal antibodies to the capsid protein of feline calicivirus (FCV). These antibodies are directed against two close but distinct epitopes, only one of which is involved in virus neutralization. We have used these antibodies and immune cat serum in immunoprecipitation and Western blotting experiments and have identified novel proteins in FCV-infected cells which we term P78, P41, P35 and P29. The number and sizes of FCV proteins now known resemble those made by other caliciviruses.

Keywords: calicivirus, protein synthesis, MAbs

Present address: Division of Immunology, New Addenbrookes Hospital, Hills Road, Cambridge CB2 2QQ, U.K.

Received 5 January 1989; accepted 13 April 1989.

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