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Barley Yellow Dwarf Virus RNA Has a 5'-Terminal Genome-linked Protein

John F. Murphy^1,

¹ Department of Plant Pathology
and² Biochemistry, University of Illinois at Urbana-Champaign, Illinois 61801, U.S.A.

The 5' terminus of barley yellow dwarf virus RNA did not become labelled with ³²P after dephosphorylation was attempted with calf intestinal alkaline phosphatase and subsequent treatment with [-³²P]ATP and T4 polynucleotide kinase. Treatment of BYDV RNA with ¹²⁵I-Bolton-Hunter reagent yielded ¹²⁵I-labelled BYDV RNA, as shown by acid precipitation analysis. Treatment with RNase yielded a protein of approximate M_r 17 000 that was destroyed by treatment with Pronase and was serologically distinct from BYDV coat protein. BYDV therefore appears to have a genome-linked protein.

Keywords: BYDV, genome-linked protein

Present address: Department of Plant Pathology, College of Agriculture, University of Kentucky, Lexington, Kentucky 40546-0091, U.S.A.

Received 6 February 1989; accepted 20 April 1989.