J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 70 (1989), 2299-2307; DOI 10.1099/0022-1317-70-9-2299
© 1989 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dietzgen, R. G.
Right arrow Articles by Jackson, A. O.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Dietzgen, R. G.
Right arrow Articles by Jackson, A. O.
Agricola
Right arrow Articles by Dietzgen, R. G.
Right arrow Articles by Jackson, A. O.

Cloning of Lettuce Necrotic Yellows Virus RNA and Identification of Virus-specific Polyadenylated RNAs in Infected Nicotiana glutinosa Leaves

R. G. Dietzgen1,2,{dagger}, B. G. Hunter2, R. I. B. Francki1 and A. O. Jackson2

1 Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond 5064, South Australia, Australia
and2 Department of Plant Pathology, University of California, Berkeley, California 94720, U.S.A.

The negative-stranded genomic RNA of lettuce necrotic yellows virus (LNYV) was isolated and estimated by denaturing agarose gel electrophoresis to consist of about 13000 nucleotides (nt). Hybridization of randomly labelled LNYV RNA to poly(A)+ RNA from infected Nicotiana glutinosa plants revealed the presence of five distinct complementary RNA species (cRNAs) ranging in size from 900 to 6450 nt. Individual recombinant cDNA clones derived from LNYV RNA hybridized to four of these cRNA species. Each cRNA contained unique sequences which together represented about 95% of the viral genome. From their size range and sequence complexity, it is assumed that the cRNAs represent messenger RNAs encoding the five LNYV structural proteins. The mRNA for the nucleocapsid protein is cRNA 3 because a recombinant cDNA expression vector clone containing sequences which hybridize to cRNA 3 produced a polypeptide which was detected with a monoclonal antibody specific for the nucleocapsid protein.

Keywords: LNYV, nucleocapsid protein, polyadenylated RNA

{dagger} Present address: Plant Pathology Branch, Queensland Department of Primary Industries, Indooroopilly, Queensland 4068, Australia.

Received 28 February 1989; accepted 18 May 1989.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1989 by the Society for General Microbiology.