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Nucleotide Sequence of the 3'-Terminal Region of Cymbidium Ringspot Virus RNA

Jòzsef Burgyan

Dipartimento di Patologia Vegetale, Via Amendola 165/A, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, 70126 Bari, Italy

The nucleotide sequences of cDNA clones corresponding to 2569 nucleotides from the 3' end of cymbidium ringspot tombusvirus (CyRSV) RNA were determined. This region contains three open reading frames giving rise to three predicted protein products, two of which had been identified in previous studies. The 3' non-coding region is 351 nucleotides long. The amino acid sequence of CyRSV coat protein has striking similarities with that of tomato bushy stunt tombusvirus, particularly in the S domain. No homology was found between the protein encoded by the second largest open reading frame and the corresponding product of other plant viruses.

Keywords: CyRSV, nucleotide sequence, 3'-terminal region

Permanent address: Research Institute for Plant Protection, P.O. Box 102, H-1525 Budapest, Hungary.

Received 8 February 1989; accepted 11 May 1989.

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