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The Nucleotide Sequences of the 3'-terminal Regions of Papaya Ringspot Virus Strains W and P

¹ Molecular Biology Research
and² Biopolymer Chemistry, The Upjohn Company, Kalamazoo, Michigan 49007
³ Department of Plant Pathology, New York State Agricultural Experiment Station, Cornell University, Geneva, New York 14456
and⁴ Plant Pathology Department, University of Florida, Gainesville, Florida 32611, U.S.A.

The sequences of cDNA clones encoding most of the NI_b protein, the coat protein and the 3' untranslated region of papaya ringspot virus (PRV) strains W and P have been determined. The open reading frame of P strain PRV was confirmed by amino acid analysis. Nucleotide sequence comparisons of these strains show that they share a 98·2% identity in their NI_b gene regions and a 97·7% identity in their coat protein genes. The sequences of these two strains are distinct from other potyvirus types, confirming their classification as two strains of the same virus. The NI_b amino acid sequence possesses conserved amino acids characteristic of RNA-dependent RNA polymerases. Comparison of the coat protein amino acid sequence with those of other potyviruses shows perfectly conserved amino acids which may have functional significance.

Received 4 July 1989; accepted 11 September 1989.

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