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Characterization of Monoclonal Antibodies that Distinguish Simian Immunodeficiency Virus Isolates from each Other and from Human Immunodeficiency Virus Types 1 and 2

¹ California Primate Research Center, University of California, Davis, California 95616
² New England Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
³ Department of Medical Pathology, School of Medicine, University of California, Davis, California 95616, U.S.A.

Two monoclonal antibodies (MAbs) against p27 and one against p17 of simian immunodeficiency virus (SIV) from rhesus macaques were produced and characterized by reacting with disrupted, viral antigens on immunoblots. Human immunodeficiency virus type 1 (HIV-1), HIV-2 and SIV isolates from sooty mangabey, stump-tailed macaque, rhesus macaque and African green monkey (SIV_SM, SIV_StM, SIV_MAC and SIV_AGM) were used for comparative analysis. The p27 monoclonal antibodies HE₃ and FA₂ reacted with SIV_MAC and SIV_SM, but not with HIV-1, HIV-2, SIV_StM and SIV_AGM. The p17 monoclonal antibodies reacted with SIV_MAC and SIV_StM, but not HIV-1, HIV-2, SIV_SM and SIV_AGM. The differential reactivity of these monoclonal antibodies indicated that common conserved antigenic epitopes are shared between SIV_MAC and SIV_SM with respect to p27 MAbs and between SIV_MAC and SIV_StM with respect to p17. Since these MAbs reacted differently with the SIV isolates, they are useful reagents for comparative pathogenesis studies for differentiating SIV isolates.

Received 13 April 1989; accepted 11 September 1989.

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