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J Gen Virol 71 (1990), 3-11; DOI 10.1099/0022-1317-71-1-3
© 1990 Society for General Microbiology
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Identification of a New Membrane-associated Polypeptide Specified by the Coronavirus Infectious Bronchitis Virus

A. R. Smith1, M. E. G. Boursnell2, M. M. Binns2, T. D. K. Brown1 and S. C. Inglis1

1 Virology Division, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
and2 Houghton Poultry Research Station, Houghton, Huntingdon, Cambridgeshire PE17 2DA, U.K.

Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with beta-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12.4K, the size expected for a D3-encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence.

Received 17 May 1989; accepted 12 September 1989.


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