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J Gen Virol 71 (1990), 2321-2329; DOI 10.1099/0022-1317-71-10-2321
© 1990 Society for General Microbiology
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Delineation of canine parvovirus T cell epitopes with peripheral blood mononuclear cells and T cell clones from immunized dogs

G. F. Rimmelzwaan1, M. C. M. Poelen1, R. H. Meloen2, J. Carlson3, F. G. C. M. UytdeHaag1 and A. D. M. E. Osterhaus1

1 Laboratory of Immunobiology, National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA Bilthoven
2 Central Veterinary Institute, P.O. Box 65, Lelystad, The Netherlands
and3 Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, U.S.A.

Three synthetic peptides derived from the amino acid sequence of VP2 of canine parvovirus (CPV) which were recently shown to represent three distinct T cell epitopes for BALB/c mice could prime BALB/c mice for a CPV-specific proliferative T cell response upon immunization. Proliferative responses of peripheral blood mononuclear cells (PBMC) from CPV-immunized dogs upon stimulation with these and other peptides, covering the major part of the sequence of VP2', identified the presence of T cell epitopes for this species. Most of these epitopes were recognized by PBMC from only a minority of the dogs tested. With three newly generated canine Thyl+ T cell clones, which recognized CPV antigen in association with major histocompatibility complex class II molecules, two distinct T cell epitopes were identified within the unique sequence of VP1.

Received 16 March 1990; accepted 28 June 1990.




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Copyright © 1990 by the Society for General Microbiology.