| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, U.K.
The complete nucleotide sequence is presented of the 2.67 kbp BamHI-EcoRV portion of the the BamHI 10 fragment of the pseudorabies virus (PRV) genome (strain Ka) containing sequences upstream of the previously reported protein kinase gene, and completing the sequence of this 4008 bp fragment. It is predicted to contain a gene designated RSp40, homologous to gene US1 of herpes simplex virus type 1 (HSV-1), with the potential to encode a protein of 364 amino acids. Analysis of PRV mRNA synthesized in the presence and absence of cycloheximide indicated that, in contrast to its HSV-1 homologue, the PRV gene RSp40 does not specify an immediate-early mRNA. Between the RSp40 gene and the protein kinase gene are two reiterated sequences: one containing 11 tandem copies of a 35 nucleotide sequence and the other containing nine tandem copies of a 10 nucleotide sequence. The BamHI 10 and the BamHI 12 fragments of PRV contain the junctions between the short unique (US) and short repeat (RS) regions of the PRV genome. The nucleotide sequence of that portion of the BamHI 12 fragment containing US sequences was determined so that, by comparison with the nucleotide sequence of the BamHI 10 fragment, the junction between the US and RS regions could be defined. In BamHI 10 this was found to be at a point between the two reiterated sequences (which are in the RS region) and the protein kinase gene (which is in the US region). The organization of this region of the PRV genome is compared to that of other alphaherpesviruses.
Received 29 May 1990;
accepted 28 June 1990.
This article has been cited by other articles:
|
|
 |
|
  H. Granzow, B. G. Klupp, and T. C. Mettenleiter The Pseudorabies Virus US3 Protein Is a Component of Primary and of Mature Virions J. Virol., February 1, 2004; 78(3): 1314 - 1323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. G. Klupp, H. Granzow, and T. C. Mettenleiter Effect of the pseudorabies virus US3 protein on nuclear membrane localization of the UL34 protein and virus egress from the nucleus J. Gen. Virol., October 1, 2001; 82(10): 2363 - 2371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Afonso, E. R. Tulman, Z. Lu, L. Zsak, D. L. Rock, and G. F. Kutish The Genome of Turkey Herpesvirus J. Virol., January 15, 2001; 75(2): 971 - 978. [Abstract] [Full Text]
|
|
|
|
|
|
E. R. Tulman, C. L. Afonso, Z. Lu, L. Zsak, D. L. Rock, and G. F. Kutish The Genome of a Very Virulent Marek's Disease Virus J. Virol., September 1, 2000; 74(17): 7980 - 7988. [Abstract] [Full Text]
|
|
|
|
|
|
W. Fuchs, C. Ehrlich, B. G. Klupp, and T. C. Mettenleiter Characterization of the replication origin (OriS) and adjoining parts of the inverted repeat sequences of the pseudorabies virus genome J. Gen. Virol., June 1, 2000; 81(6): 1539 - 1543. [Abstract] [Full Text]
|
|
|
|
|
|
W. A. Derbigny, S. K. Kim, G. B. Caughman, and D. J. O'Callaghan The EICP22 Protein of Equine Herpesvirus 1 Physically Interacts with the Immediate-Early Protein and with Itself To Form Dimers and Higher-Order Complexes J. Virol., February 1, 2000; 74(3): 1425 - 1435. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. A. Smith and L. W. Enquist A self-recombining bacterial artificial chromosome and its application for analysis of herpesvirus pathogenesis PNAS, April 25, 2000; 97(9): 4873 - 4878. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
