J Gen Virol
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J Gen Virol 71 (1990), 2543-2551; DOI 10.1099/0022-1317-71-11-2543
© 1990 Society for General Microbiology
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Antigenic structure of chimeras of type 1 and type 3 poliovirus involving antigenic site 1

P. D. Minor1, M. Ferguson1, K. Katrak1, D. Wood1, A. John1, J. Howlett1, G. Dunn1, K. Burke2 and J. W. Almond2

1 National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG
and2 Department of Microbiology, University of Reading, London Road, Reading RG1 5AQ, U.K.

Chimeric polioviruses have been prepared in which part of the antigenic site 1-encoding sequence of the Sabin strain of type 1 poliovirus has been replaced by sequences based on those found in the homologous region of the Sabin type 3 strain. The chimeras were analysed for their reaction with polyclonal and monoclonal antibodies raised against type 1 and type 3 viruses, and with polyclonal antipeptide sera, as well as for their immunogenicity in animals. The effectiveness with which the type 3 site was presented antigenically varied in ways which were partially predictable, based on the behaviour of type 3 mutants with monoclonal antibodies. However, other factors were implicated which may include conformational effects and other components of the site in addition to those altered in the chimeras. The ability of the chimeras to induce antibodies reacting with type 3 polioviruses paralleled their antigenic reactivity, and evidence is presented for the induction of strain-specific antibodies.

Received 12 June 1990; accepted 27 July 1990.


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