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J Gen Virol 71 (1990), 2657-2664; DOI 10.1099/0022-1317-71-11-2657
© 1990 Society for General Microbiology
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Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps

Jean-Robert Bonami1, Bronwen Trumper2, Jocelyne Mari1, Michel Brehelin1 and Donald V. Lightner2

1 ERPAM, Laboratoire de Pathologie Comparée, INRA-CNRS, Université des Sciences et Techniques du Languedoc, Place E. Bataillon, 34095 Montpellier Cedex 5, France
and2 Department of Veterinary Science, University of Arizona, Tucson, Arizona 85721, U.S.A.

Infectious hypodermal and haematopoietic necrosis (IHHN) is one of the most important viral diseases of cultured penaeid shrimps and is potentially a limiting factor in the development of farming projects for some species of these shrimps. Although the IHHN agent was recognized early as being viral in origin, attempts to characterize it were inconclusive because of difficulties in obtaining sufficient amounts of purified virions to permit its characterization. Recent improvements of purification procedures have allowed the physicochemical characterization of this virus. Purified IHHNV is a non-enveloped icosahedral particle averaging 22 nm in diameter, exhibiting a mean buoyant density of 1.40 g/ml in CsCl. The genome is a single molecule of ssDNA with an estimated size of 4.1 kb by molecule length measurement in transmission electron microscopy. As determined by SDS-PAGE, the particle contains four polypeptides with Mrs of 74K, 47K, 39K and 37.5K, respectively. From its characteristics, this virus could be a member of the Parvoviridae family.

Received 5 April 1990; accepted 16 July 1990.




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Copyright © 1990 by the Society for General Microbiology.