| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE
and2 MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K.
Studies with mutant viruses have suggested that the product of gene UL41 of herpes simplex virus type 1 (HSV-1) controls the virion-mediated inhibition of cellular protein synthesis as well as the rate of degradation of viral mRNAs. HSV-1 strain 17+ has a weak host shutoff function, whereas HSV-2 strain G shuts off strongly. A gene of HSV-2(G), judged from its position in the genome to be the probable analogue of gene UL41 of HSV-1, was inserted into the non-essential thymidine kinase gene of HSV-1(17+). The recombinant virus, 17G41, exhibited a strong shutoff function and its immediate early mRNA did not accumulate in the presence of cycloheximide. It resembled HSV-2(G) in these respects and not the parent, confirming the function of the transferred gene. Recombinant virus 17G41 carries the UL41 genes of both strains, 17+ and G, and in this situation the strong shutoff function was dominant. However, after mixed infection with equal multiplicities of 17G41 and HSV-1(17+) the weak shutoff function was dominant. The recombinant, 17G41, was further modified by insertion of a lacZ expression cassette into the coding region of the original gene UL41 (17+). The resulting virus, 17(41-)G41, also had a strong shutoff activity but grew poorly in tissue culture.
Received 7 June 1989;
accepted 17 October 1989.
This article has been cited by other articles:
|
|
 |
|
  N. Sarma, D. Agarwal, L. A. Shiflett, and G. S. Read Small Interfering RNAs That Deplete the Cellular Translation Factor eIF4H Impede mRNA Degradation by the Virion Host Shutoff Protein of Herpes Simplex Virus J. Virol., July 1, 2008; 82(13): 6600 - 6609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Doepker, W.-L. Hsu, H. A. Saffran, and J. R. Smiley Herpes Simplex Virus Virion Host Shutoff Protein Is Stimulated by Translation Initiation Factors eIF4B and eIF4H J. Virol., May 1, 2004; 78(9): 4684 - 4699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Murphy, R. J. Duerst, T. J. Smith, and L. A. Morrison Herpes Simplex Virus Type 2 Virion Host Shutoff Protein Regulates Alpha/Beta Interferon but Not Adaptive Immune Responses during Primary Infection In Vivo J. Virol., September 1, 2003; 77(17): 9337 - 9345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sato, L. D. Callanan, L. Pesnicak, T. Krogmann, and J. I. Cohen Varicella-Zoster Virus (VZV) ORF17 Protein Induces RNA Cleavage and Is Critical for Replication of VZV at 37oC but Not 33oC J. Virol., November 1, 2002; 76(21): 11012 - 11023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Lu, F. E. Jones, H. A. Saffran, and J. R. Smiley Herpes Simplex Virus Virion Host Shutoff Protein Requires a Mammalian Factor for Efficient In Vitro Endoribonuclease Activity J. Virol., February 1, 2001; 75(3): 1172 - 1185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Lu, H. A. Saffran, and J. R. Smiley The vhs1 Mutant Form of Herpes Simplex Virus Virion Host Shutoff Protein Retains Significant Internal Ribosome Entry Site-Directed RNA Cleavage Activity J. Virol., January 15, 2001; 75(2): 1072 - 1076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Geiss, T. J. Smith, D. A. Leib, and L. A. Morrison Disruption of Virion Host Shutoff Activity Improves the Immunogenicity and Protective Capacity of a Replication-Incompetent Herpes Simplex Virus Type 1 Vaccine Strain J. Virol., December 1, 2000; 74(23): 11137 - 11144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Smith, C. E. Ackland-Berglund, and D. A. Leib Herpes Simplex Virus Virion Host Shutoff (vhs) Activity Alters Periocular Disease in Mice J. Virol., April 15, 2000; 74(8): 3598 - 3604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Everly Jr. and G. S. Read Site-Directed Mutagenesis of the Virion Host Shutoff Gene (UL41) of Herpes Simplex Virus (HSV): Analysis of Functional Differences between HSV Type 1 (HSV-1) and HSV-2 Alleles J. Virol., November 1, 1999; 73(11): 9117 - 9129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Elgadi, C. E. Hayes, and J. R. Smiley The Herpes Simplex Virus vhs Protein Induces Endoribonucleolytic Cleavage of Target RNAs in Cell Extracts J. Virol., September 1, 1999; 73(9): 7153 - 7164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Grassie, D McNab, and J. Macnab The characteristic which makes the cell-coded HSV-inducible U90 distinctive in transformed cells is its greatly increased half-life J. Cell Sci., January 4, 1993; 104(4): 1083 - 1090. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
