| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Northern Ireland Centre for Genetic Engineering, The Queen's University of Belfast, Belfast BT9 7BL, Northern Ireland
and2 Statens Bakteriologiska Laboratoriet, S-105 21 Stockholm, Sweden
Evidence has been presented that generation of polycistronic readthrough RNAs in mumps virus-infected cells is not a simple stochastic process with strain-dependent variations in the generation of certain readthrough products, but that this process is affected by host as well as viral factors. RNAs extracted from infected Vero cells or chicken embryo fibroblast (CEF) cells have been analysed by Northern blotting with virus-specific probes for the nucleocapsid (N), phosphoprotein (P), matrix (M), fusion (F), small hydrophobic (SH) and haemagglutinin—neuraminidase (HN) genes. Vero cells infected with tissue culture cell-adapted virus strains generate monocistronic as well as polycistronic RNAs. Transcription analysis of Vero cells infected with an egg-adapted strain reveal the absence of monocistronic M and F transcripts, with a concomitant increase in readthrough transcripts involving these genes. When the same virus infects CEF cells monocistronic RNAs accumulate. The presence of viral proteins in the various virus/host cell combinations assessed by immunofluorescence with mumps virus-specific monoclonal antibodies for the N, P, M, F and HN proteins correlates well with the patterns of transcription.
Received 6 July 1989;
accepted 6 November 1989.
This article has been cited by other articles:
|
|
 |
|
  P. Chambers, B. K. Rima, and W. P. Duprex Molecular differences between two Jeryl Lynn mumps virus vaccine component strains, JL5 and JL2 J. Gen. Virol., December 1, 2009; 90(12): 2973 - 2981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. B. Sharma, S. Ohgimoto, S. Kato, S. Kurazono, M. Ayata, K. Takeuchi, T. Ihara, and H. Ogura Contribution of Matrix, Fusion, Hemagglutinin, and Large Protein Genes of the CAM-70 Measles Virus Vaccine Strain to Efficient Growth in Chicken Embryonic Fibroblasts J. Virol., November 15, 2009; 83(22): 11645 - 11654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Palacios, O. Jabado, D. Cisterna, F. de Ory, N. Renwick, J. E. Echevarria, A. Castellanos, M. Mosquera, M. C. Freire, R. H. Campos, et al. Molecular Identification of Mumps Virus Genotypes from Clinical Samples: Standardized Method of Analysis J. Clin. Microbiol., April 1, 2005; 43(4): 1869 - 1878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Magrangeas, G. Pitiot, S. Dubois, E. Bragado-Nilsson, M. Cherel, S. Jobert, B. Lebeau, O. Boisteau, B. Lethe, J. Mallet, et al. Cotranscription and Intergenic Splicing of Human Galactose-1-phosphate Uridylyltransferase and Interleukin-11 Receptor alpha -Chain Genes Generate a Fusion mRNA in Normal Cells. IMPLICATION FOR THE PRODUCTION OF MULTIDOMAIN PROTEINS DURING EVOLUTION J. Biol. Chem., June 26, 1998; 273(26): 16005 - 16010. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
