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Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville, Florida 32610, U.S.A.
We have developed a cell-free system derived from measles virus-infected cells that supported the transcription and replication of measles virus RNA in vitro. The data suggest that tubulin may be required for these reactions, since an anti-
-tubulin monoclonal antibody inhibited viral RNA synthesis and the addition of purified tubulin stimulated measles virus RNA synthesis in vitro. Tubulin may be a subunit of the viral RNA polymerase, since two different anti-tubulin antibodies, one specific for the - and another specific for the 
-subunit of tubulin, coimmunoprecipitated the measles virus L protein as well as tubulin from extracts of measles virus-infected cells. Other experiments further implicated actin in the budding process during virus maturation, as there appeared to be a specific association of actin in vitro only with nucleocapsids that have terminated RNA synthesis, which is presumably a prerequisite to budding.
Present address: Department of Microbiology, University of Southern California School of Medicine, Los Angeles, California 90033, U.S.A.
Received 7 June 1989;
accepted 13 December 1989.
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