J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


J Gen Virol 71 (1990), 819-823; DOI 10.1099/0022-1317-71-4-819
© 1990 Society for General Microbiology

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Todd, D.
Right arrow Articles by McNulty, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Todd, D.
Right arrow Articles by McNulty, M. S.
Agricola
Right arrow Articles by Todd, D.
Right arrow Articles by McNulty, M. S.

Purification and biochemical characterization of chicken anaemia agent

Daniel Todd1, Julie L. Creelan1, Dermot P. Mackie1, Frazer Rixon2 and M. Stewart McNulty1

1 Virology Section, Veterinary Research Laboratories, Stormont, Belfast BT4 3SD
and2 Institute of Virology, Church Street, Glasgow G11 5JR, U.K.

Chicken anaemia agent (CAA) was purified using differential centrifugation and successive cycles of equilibrium density gradient centrifugation using sucrose and CsCl. The purification method was dependent on the use of an antigen-detecting ELISA based on a CAA-specific monoclonal antibody. Virus particles banded at a density of 1.33 to 1.34 g/ml in CsCl and measured 23.5 ± 0.8 nm in diameter. Purified preparations contained one major polypeptide (Mr 50 000) and a single-stranded, circular DNA (2.3 kb). CAA shares some of the biochemical characteristics possessed by porcine circovirus and the virus associated with psittacine beak and feather disease.

Received 18 October 1989; accepted 13 December 1989.


This article has been cited by other articles:


Home page
J. Gen. Virol.Home page
M. A. Peters, B. S. Crabb, K. A. Tivendale, and G. F. Browning
Attenuation of chicken anemia virus by site-directed mutagenesis of VP2
J. Gen. Virol., August 1, 2007; 88(8): 2168 - 2175.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
K. Kamada, A. Kuroishi, T. Kamahora, P. Kabat, S. Yamaguchi, and S. Hino
Spliced mRNAs detected during the life cycle of Chicken anemia virus.
J. Gen. Virol., August 1, 2006; 87(Pt 8): 2227 - 2233.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
M. M. Miller, K. W. Jarosinski, and K. A. Schat
Positive and Negative Regulation of Chicken Anemia Virus Transcription
J. Virol., March 1, 2005; 79(5): 2859 - 2868.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
R. A. Crowther, J. A. Berriman, W. L. Curran, G. M. Allan, and D. Todd
Comparison of the Structures of Three Circoviruses: Chicken Anemia Virus, Porcine Circovirus Type 2, and Beak and Feather Disease Virus
J. Virol., December 15, 2003; 77(24): 13036 - 13041.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
D. Todd, A. N. J. Scott, N. W. Ball, B. J. Borghmans, and B. M. Adair
Molecular Basis of the Attenuation Exhibited by Molecularly Cloned Highly Passaged Chicken Anemia Virus Isolates
J. Virol., July 17, 2002; 76(16): 8472 - 8474.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
L. Kakkola, K. Hedman, H. Vanrobaeys, L. Hedman, and M. Soderlund-Venermo
Cloning and sequencing of TT virus genotype 6 and expression of antigenic open reading frame 2 proteins
J. Gen. Virol., May 1, 2002; 83(5): 979 - 990.
[Abstract] [Full Text] [PDF]


Home page
Clin. Microbiol. Rev.Home page
M. Bendinelli, M. Pistello, F. Maggi, C. Fornai, G. Freer, and M. L. Vatteroni
Molecular Properties, Biology, and Clinical Implications of TT Virus, a Recently Identified Widespread Infectious Agent of Humans
Clin. Microbiol. Rev., January 1, 2001; 14(1): 98 - 113.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
P. Nawagitgul, I. Morozov, S. R. Bolin, P. A. Harms, S. D. Sorden, and P. S. Paul
Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein
J. Gen. Virol., September 1, 2000; 81(9): 2281 - 2287.
[Abstract] [Full Text]


Home page
J. Gen. Virol.Home page
D. Mahé, P. Blanchard, C. Truong, C. Arnauld, P. Le Cann, R. Cariolet, F. Madec, E. Albina, and A. Jestin
Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes
J. Gen. Virol., July 1, 2000; 81(7): 1815 - 1824.
[Abstract] [Full Text]


Home page
J. Clin. Microbiol.Home page
M. Ouardani, L. Wilson, R. Jetté, C. Montpetit, and S. Dea
Multiplex PCR for Detection and Typing of Porcine Circoviruses
J. Clin. Microbiol., December 1, 1999; 37(12): 3917 - 3924.
[Abstract] [Full Text]


Home page
J. Clin. Microbiol.Home page
I. Morozov, T. Sirinarumitr, S. D. Sorden, P. G. Halbur, M. K. Morgan, K.-J. Yoon, and P. S. Paul
Detection of a Novel Strain of Porcine Circovirus in Pigs with Postweaning Multisystemic Wasting Syndrome
J. Clin. Microbiol., September 1, 1998; 36(9): 2535 - 2541.
[Abstract] [Full Text]


Home page
J. Virol.Home page
A. L. Hamel, L. L. Lin, and G. P. S. Nayar
Nucleotide Sequence of Porcine Circovirus Associated with Postweaning Multisystemic Wasting Syndrome in Pigs
J. Virol., June 1, 1998; 72(6): 5262 - 5267.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
A Mankertz, J Mankertz, K Wolf, and H. Buhk
Identification of a protein essential for replication of porcine circovirus
J. Gen. Virol., February 1, 1998; 79(2): 381 - 384.
[Abstract]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1990 by the Society for General Microbiology.