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1 Virology Section, Veterinary Research Laboratories, Stormont, Belfast BT4 3SD
and2 Institute of Virology, Church Street, Glasgow G11 5JR, U.K.
Chicken anaemia agent (CAA) was purified using differential centrifugation and successive cycles of equilibrium density gradient centrifugation using sucrose and CsCl. The purification method was dependent on the use of an antigen-detecting ELISA based on a CAA-specific monoclonal antibody. Virus particles banded at a density of 1.33 to 1.34 g/ml in CsCl and measured 23.5 ± 0.8 nm in diameter. Purified preparations contained one major polypeptide (Mr 50 000) and a single-stranded, circular DNA (2.3 kb). CAA shares some of the biochemical characteristics possessed by porcine circovirus and the virus associated with psittacine beak and feather disease.
Received 18 October 1989;
accepted 13 December 1989.
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