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In vitro synthesis of biologically active transcripts of tomato black ring virus satellite RNA

Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du Général Zimmer, 67000 Strasbourg, France

Synthetic transcripts of tomato black ring virus satellite RNA (TBRV satRNA), isolate L, were prepared from cDNA cloned in the Bluescribe transcription vector. Transcripts with 49 (T49L) or two (T2GL) extra nucleotides at their 5' ends and 42 extra nucleotides at their 3' ends were able to induce, but to different extents, the synthesis in vitro of the satRNA-encoded 48K protein. However, when inoculated into Chenopodium quinoa together with TBRV L genomic RNAs, only T2GL was biologically active, in the presence or absence of a 5' cap analogue in the transcription reactions. Analysis of the 5' and 3' termini of the satRNA isolated from plants showed that non-viral extensions were not maintained in the transcript progeny.

Received 11 October 1989; accepted 11 December 1989.