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J Gen Virol 71 (1990), 945-948; DOI 10.1099/0022-1317-71-4-945
© 1990 Society for General Microbiology
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Double-stranded RNAs isolated from plant tissue infected with red clover necrotic mosaic virus correspond to genomic and subgenomic single-stranded RNAs

T. A. M. Osman and K. W. Buck

Department of Pure and Applied Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB, U.K.

Red clover necrotic mosaic dianthovirus (RCNMV) has a genome of two ssRNA species, RNA 1 (4.0 kb) and RNA 2 (1.4 kb). Double-stranded RNA was isolated from Phaseolus vulgaris plants infected separately with five isolates of RCNMV from Czechoslovakia, Sweden, Scotland and England. In each case three species of dsRNA, designated A, B and C in order of increasing mobility, were resolved by gel electrophoresis in non-denaturing conditions. The mobilities of dsRNA species A and B were similar for all the isolates, but the mobilities of the different species of dsRNA C showed some variation. Southern blotting followed by hybridization with cloned probes showed that dsRNA species A and B are related to RNA 1, whereas dsRNA C is related to RNA 2. In gel electrophoresis in denaturing conditions, RNA from dsRNA A had the same mobility as ssRNA 1, whereas RNA from dsRNA species B and C had the same mobility as ssRNA 2. It is concluded that dsRNAs A and C are the doublestranded forms of the genomic RNA 1 and RNA 2 respectively and that dsRNA B is the double-stranded form of a subgenomic RNA which is derived from RNA 1 and has the same size as RNA 2.

Received 11 September 1989; accepted 22 December 1989.




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