| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institute of Virology, University of Würzburg, Versbacher Strasse 7, 8700 Würzburg, F.R.G.
The gene encoding the spike glycoprotein of the human coronavirus HCV 229E has been cloned and sequenced. This analysis predicts an S polypeptide of 1173 amino acids with an Mr of 128600. The polypeptide has 30 potential N-glycosylation sites. A number of structural features typical of coronavirus S proteins can be recognized, including a signal sequence, a membrane anchor, heptad repeat structures and a carboxy-terminal cysteine cluster. A detailed, computer-aided comparison with the S proteins of infectious bronchitis virus, feline infectious peritionitis virus, transmissible gastroenteritis virus and murine hepatitis virus, strain JHM is presented. We have also done a Northern blot analysis of viral RNAs in HCV 229E-infected cells using synthetic oligonucleotides. On the basis of this analysis, and by analogy to the replication strategy of other coronaviruses, we are able to propose a model for the organization and expression of the HCV 229E genome.
Received 9 November 1989;
accepted 18 January 1990.
This article has been cited by other articles:
|
|
 |
|
  A. Putics, W. Filipowicz, J. Hall, A. E. Gorbalenya, and J. Ziebuhr ADP-Ribose-1"-Monophosphatase: a Conserved Coronavirus Enzyme That Is Dispensable for Viral Replication in Tissue Culture J. Virol., October 15, 2005; 79(20): 12721 - 12731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Ivanov and J. Ziebuhr Human Coronavirus 229E Nonstructural Protein 13: Characterization of Duplex-Unwinding, Nucleoside Triphosphatase, and RNA 5'-Triphosphatase Activities J. Virol., July 15, 2004; 78(14): 7833 - 7838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bonavia, B. D. Zelus, D. E. Wentworth, P. J. Talbot, and K. V. Holmes Identification of a Receptor-Binding Domain of the Spike Glycoprotein of Human Coronavirus HCoV-229E J. Virol., February 15, 2003; 77(4): 2530 - 2538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Thiel, J. Herold, B. Schelle, and S. G. Siddell Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus J. Gen. Virol., June 1, 2001; 82(6): 1273 - 1281. [Abstract] [Full Text]
|
|
|
|
|
|
G. Wang, C. Deering, M. Macke, J. Shao, R. Burns, D. M. Blau, K. V. Holmes, B. L. Davidson, S. Perlman, and P. B. McCray Jr. Human Coronavirus 229E Infects Polarized Airway Epithelia from the Apical Surface J. Virol., October 1, 2000; 74(19): 9234 - 9239. [Abstract] [Full Text]
|
|
|
|
|
|
N. Arbour, S. Ekandé, G. Côté, C. Lachance, F. Chagnon, M. Tardieu, N. R. Cashman, and P. J. Talbot Persistent Infection of Human Oligodendrocytic and Neuroglial Cell Lines by Human Coronavirus 229E J. Virol., April 1, 1999; 73(4): 3326 - 3337. [Abstract] [Full Text]
|
|
|
|
|
|
C. Lachance, N. Arbour, N. R. Cashman, and P. J. Talbot Involvement of Aminopeptidase N (CD13) in Infection of Human Neural Cells by Human Coronavirus 229E J. Virol., August 1, 1998; 72(8): 6511 - 6519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Herold, A. E. Gorbalenya, V. Thiel, B. Schelle, and S. G. Siddell Proteolytic Processing at the Amino Terminus of Human Coronavirus 229E Gene 1-Encoded Polyproteins: Identification of a Papain-Like Proteinase and Its Substrate J. Virol., February 1, 1998; 72(2): 910 - 918. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
