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Pseudorabies Virus Glycoprotein gI: in Vitro and in Vivo Analysis of Immunorelevant Epitopes

Noèmi Lukàcs^1,

¹ Federal Research Centre for Virus Diseases of Animals, P.O. Box 1149, D-7400 Tübingen, F.R.G.
and² Intervet International BV, P.O. Box 31, NL-5830 AA Boxmeer, The Netherlands

Overlapping fragments of the gene encoding glycoprotein gI of pseudorabies virus (PRV; herpesvirus suis 1) were expressed in bacteria. Using the fusion proteins and a panel of monoclonal antibodies (MAbs) against gI as well as swine sera we found that the N-terminal part of gI (residues 33 to approximately 100) contains a highly antigenic and immunogenic domain. Transfer of antibodies binding to this region as well as vaccination with fusion proteins containing the N terminus of gI are able to confer protection to mice against a lethal challenge of virus. The results show that gI, which is non-essential for virus replication in tissue culture, can induce neutralizing and protective antibodies. The potential suitability of fusion proteins encompassing N-terminal parts of gI as diagnostic tools is demonstrated.

Present address: Universität Düsseldorf, Institut für Physikalische Biologie, Universitätsstrasse 1, D-4000 Düsseldorf, F.R.G.

Received 6 November 1989; accepted 31 January 1990.

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