HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Precious, B. Articles by Randall, R. E. Search for Related Content PubMed PubMed Citation Articles by Precious, B. Articles by Randall, R. E. Agricola Articles by Precious, B. Articles by Randall, R. E.

Sequence Analysis of the HN Gene of Parainfluenza Virus Type 2

Department of Biochemistry and Microbiology, University of St Andrews, North Street, St Andrews, Fife KY16 9AL, U.K.

A cDNA library was constructed in gt10 using mRNA purified from cells infected with parainfluenza virus type 2 (PIV2). Virus-specific clones were identified by screening the library with ³²P-labelled cDNA probes made from randomly primed vRNA. Clones containing the haemagglutinin—neuraminidase (HN) gene were identified by sequence comparisons with known para-influenza virus HN gene sequences. The largest HN clone isolated had a nucleic acid sequence of 2065 bp with a single long open reading frame encoding a protein of 571 amino acids. The HN protein has nine predicted glycosylation sites and an amino-terminal membrane-spanning region. The PIV2 HN protein shares 43% amino acid identity with the HN protein of simian virus 5 and 40% with mumps virus, 30% of the amino acids being common to all three viruses.

Received 14 November 1989; accepted 3 January 1990.