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Cytopathic and Non-cytopathic Biotypes of Border Disease Virus Induce Polypeptides of Different Molecular Weight with Common Antigenic Determinants

¹ Department of Veterinary Pathology, University of Edinburgh, Summerhall, Edinburgh EH9 1QH
and² Moredun Research Institute, 408 Gilmerton Road, Edinburgh EH17 7JH, U.K.

Ten monoclonal antibodies have been raised against lysates of cells infected with cytopathic border disease virus (BDV). These antibodies all recognize non-cytopathic BDV and react with a number of different strains of bovine viral diarrhoea virus (BVDV). Studies with radiolabelled cell lysates show that all the antibodies precipitate two polypeptides of apparent M_r 80000 and 130000 from cells infected with cytopathic virus and a single polypeptide of apparent M_r 120000 from cells infected with non-cytopathic virus. Two of the monoclonal antibodies react on immunoblots and show the same pattern of reactivity indicating that these three polypeptides are antigenically related.

Received 22 September 1989; accepted 9 February 1990.