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Physical map of Hz-1 baculovirus genome from standard and defective interfering particles

Yu-Chan Chao

Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, New York 14853, U.S.A.

Restriction maps of the 228 kb genome of the Hz-1 standard baculovirus were constructed for XhoI, HindIII, EcoRI, SstII and SmaI, using cosmid pVK102 and pBluescript vectors. The genome does not contain a NotI restriction site. Three regions of the genome were unclonable and were mapped by isolation of DNA fragments, in vitro labelling and Southern hybridization procedures. Serial passage in tissue culture was used to produce defective interfering (DI) particles. The majority of DI particles in five virus isolates contained genomic deletions ranging from 24 to 52 kb in the 22 to 45 map unit region.

Present address: Institute of Molecular Biology, Academia Sinica, Nanking, Taipei, Taiwan, Republic of China.

Received 15 August 1989; accepted 22 February 1990.