| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Medical Research Council Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K.
Two sites within the short region origin of DNA replication (oriS) in herpes simplex virus type 1 (HSV-1) which bind the product of the UL9 gene have previously been identified. One of these sites (site I) contains an 11 bp sequence which is also present in oriS of varicella-zoster virus, and the other (site II) includes a related element differing in two positions. A third sequence (motif III), which lies close to binding site I, differs from the site I element at only a single position. We have deleted specifically each of these three 11 bp sequences from within functional copies of HSV-1 oriS and have examined the effects on origin activity and binding of the UL9 protein. Gel retardation analyses confirmed the important roles of the regions deleted from sites I and II in interacting with the UL9 protein. In transient replication assays, copies of oriS lacking the site I or II elements exhibited undetectable or residual (4 to 8%) activity respectively. The UL9 protein did not bind to motif III even in the absence of site I sequences, although removal of the motif III sequence caused a small reduction in oriS activity. A single base change which converted the sequence within binding site I to that of motif III was sufficient to abolish both the interaction of the UL9 gene product at this locus and the replicative ability of oriS. Therefore, interaction of the UL9 protein with binding site I is essential for origin activity, but the presence of binding site II is also required for efficient replication.
Received 18 December 1989;
accepted 22 February 1990.
This article has been cited by other articles:
|
|
 |
|
  S. Chattopadhyay and S. K. Weller DNA Binding Activity of the Herpes Simplex Virus Type 1 Origin Binding Protein, UL9, Can Be Modulated by Sequences in the N Terminus: Correlation between Transdominance and DNA Binding J. Virol., May 1, 2006; 80(9): 4491 - 4500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Balliet, J. C. Min, M. S. Cabatingan, and P. A. Schaffer Site-Directed Mutagenesis of Large DNA Palindromes: Construction and In Vitro Characterization of Herpes Simplex Virus Type 1 Mutants Containing Point Mutations That Eliminate the oriL or oriS Initiation Function J. Virol., October 15, 2005; 79(20): 12783 - 12797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Colletti, Y. Xu, I. Yamboliev, and G. S. Pari Human Cytomegalovirus UL84 Is a Phosphoprotein That Exhibits UTPase Activity and Is a Putative Member of the DExD/H Box Family of Proteins J. Biol. Chem., March 25, 2005; 280(12): 11955 - 11960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, S. A. Cei, A. Rodriguez Huete, K. S. Colletti, and G. S. Pari Human Cytomegalovirus DNA Replication Requires Transcriptional Activation via an IE2- and UL84-Responsive Bidirectional Promoter Element within oriLyt J. Virol., November 1, 2004; 78(21): 11664 - 11677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aslani, M. Olsson, and P. Elias ATP-dependent Unwinding of a Minimal Origin of DNA Replication by the Origin-binding Protein and the Single-strand DNA-binding Protein ICP8 from Herpes Simplex Virus Type I J. Biol. Chem., October 18, 2002; 277(43): 41204 - 41212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, K. S. Colletti, and G. S. Pari Human Cytomegalovirus UL84 Localizes to the Cell Nucleus via a Nuclear Localization Signal and Is a Component of Viral Replication Compartments J. Virol., July 29, 2002; 76(17): 8931 - 8938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Isler and P. A. Schaffer Origin Binding Protein-Containing Protein-DNA Complex Formation at Herpes Simplex Virus Type 1 oriS: Role in oriS-Dependent DNA Replication J. Virol., August 1, 2001; 75(15): 6808 - 6816. [Abstract] [Full Text]
|
|
|
|
|
|
F. Y. Wu, J.-H. Ahn, D. J. Alcendor, W.-J. Jang, J. Xiao, S. D. Hayward, and G. S. Hayward Origin-Independent Assembly of Kaposi's Sarcoma-Associated Herpesvirus DNA Replication Compartments in Transient Cotransfection Assays and Association with the ORF-K8 Protein and Cellular PML J. Virol., February 1, 2001; 75(3): 1487 - 1506. [Abstract] [Full Text]
|
|
|
|
 |
|
 J Nicholas Evolutionary aspects of oncogenic herpesviruses Mol. Pathol., October 1, 2000; 53(5): 222 - 237. [Abstract] [Full Text]
|
|
|
|
|
|
M. N. Prichard, S. Jairath, M. E. T. Penfold, S. St. Jeor, M. C. Bohlman, and G. S. Pari Identification of Persistent RNA-DNA Hybrid Structures within the Origin of Replication of Human Cytomegalovirus J. Virol., September 1, 1998; 72(9): 6997 - 7004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Fierer and D. S. Fierer The Stoichiometry of Binding of the Herpes Simplex Virus Type 1 Origin Binding Protein, UL9, to Ori(S) J. Biol. Chem., March 31, 1995; 270(13): 7330 - 7334. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
