HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Serghini, M. A. Articles by Pinck, L. Search for Related Content PubMed PubMed Citation Articles by Serghini, M. A. Articles by Pinck, L. Agricola Articles by Serghini, M. A. Articles by Pinck, L.

RNA2 of grapevine fanleaf virus: sequence analysis and coat protein cistron location

¹ Institut de Biologie Moléculaire des Plantes du CNRS et Université Louis Pasteur, Laboratoire de Virologie, 12 rue du Général Zimmer, 67084 Strasbourg
² Station de Recherches Vigne et Vin, Laboratoire de Pathologie Végétale INRA, 28 rue de Herrlisheim, 68021 Colmar
and³ Institut de Biologie Moléculaire et Cellulaire du CNRS, 15 rue Descartes, 67084 Strasbourg, France

The nucleotide sequence of the genomic RNA2 (3774 nucleotides) of grapevine fanleaf virus strain F13 was determined from overlapping cDNA clones and its genetic organization was deduced. Two rapid and efficient methods were used for cDNA cloning of the 5' region of RNA2. The complete sequence contained only one long open reading frame of 3555 nucleotides (1184 codons, 131K product). The analysis of the N-terminal sequence of purified coat protein (CP) and identification of its C-terminal residue have allowed the CP cistron to be precisely positioned within the polyprotein. The CP produced by proteolytic cleavage at the Arg/Gly site between residues 680 and 681 contains 504 amino acids (M_r 56019) and has hydrophobic properties. The Arg/Gly cleavage site deduced by N-terminal amino acid sequence analysis is the first for a nepovirus coat protein and for plant viruses expressing their genomic RNAs by polyprotein synthesis. Comparison of GFLV RNA2 with M RNA of cowpea mosaic comovirus and with RNA2 of two closely related nepoviruses, tomato black ring virus and Hungarian grapevine chrome mosaic virus, showed strong similarities among the 3' non-coding regions but less similarity among the 5' end non-coding sequences than reported among other nepovirus RNAs.

Received 4 January 1990; accepted 12 March 1990.

This article has been cited by other articles:

				E. Vigne, M. Bergdoll, S. Guyader, and M. Fuchs Population structure and genetic variability within isolates of Grapevine fanleaf virus from a naturally infected vineyard in France: evidence for mixed infection and recombination J. Gen. Virol., August 1, 2004; 85(8): 2435 - 2445. [Abstract] [Full Text] [PDF]

				J. R. Thompson, G. Leone, J. L. Lindner, W. Jelkmann, and C. D. Schoen Characterization and complete nucleotide sequence of Strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses J. Gen. Virol., January 1, 2002; 83(1): 229 - 239. [Abstract] [Full Text] [PDF]

				P. Naraghi-Arani, S. Daubert, and A. Rowhani Quasispecies nature of the genome of Grapevine fanleaf virus J. Gen. Virol., July 1, 2001; 82(7): 1791 - 1795. [Abstract] [Full Text] [PDF]

				C. Li, N. Yoshikawa, T. Takahashi, T. Ito, K. Yoshida, and H. Koganezawa Nucleotide sequence and genome organization of Apple latent spherical virus: a new virus classified into the family Comoviridae J. Gen. Virol., February 1, 2000; 81(2): 541 - 547. [Abstract] [Full Text]

				C Belin, C Schmitt, F Gaire, B Walter, G Demangeat, and L Pinck The nine C-terminal residues of the grapevine fanleaf nepovirus movement protein are critical for systemic virus spread J. Gen. Virol., June 1, 1999; 80(6): 1347 - 1356. [Abstract]