HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Klippmark, E. Articles by Norrby, E. Search for Related Content PubMed PubMed Citation Articles by Klippmark, E. Articles by Norrby, E. Agricola Articles by Klippmark, E. Articles by Norrby, E.

Antigenic variation of human and bovine parainfluenza virus type 3 strains

Department of Virology, Karolinska Institute, School of Medicine, c/o SBL, S-105 21 Stockholm, Sweden
and¹ Department of Viral Infection, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan

Three human and six bovine parainfluenza virus type 3 (PIV3) strains were examined by the use of 60 monoclonal antibodies (MAbs). Fifty-three MAbs to the human C243 strain were directed against six, four, nine and seven epitopes of the haemagglutinin—neuraminidase (HN), fusion (F), nucleocapsid (N) and matrix proteins, respectively. Seven MAbs to the bovine strain were directed against three epitopes of the HN protein and three epitopes of the F protein. Each strain was characterized in ELISA and immunofluorescence tests with all MAbs and in a haemagglutination inhibition assay with the anti-HN MAbs. There were marked differences between human and bovine viruses, primarily in the HN protein where five epitopes differed. One epitope of the F and one of the N protein also differed. Bovine PIV3 was found to be a homogeneous subtype and distinct from human PIV3.

Received 4 January 1990; accepted 7 March 1990.

This article has been cited by other articles:

				S. Pennathur, A. A. Haller, M. MacPhail, T. Rizzi, S. Kaderi, F. Fernandes, L. Bicha, J. H. Schickli, R. S. Tang, W. Chen, et al. Evaluation of attenuation, immunogenicity and efficacy of a bovine parainfluenza virus type 3 (PIV-3) vaccine and a recombinant chimeric bovine/human PIV-3 vaccine vector in rhesus monkeys J. Gen. Virol., December 1, 2003; 84(12): 3253 - 3261. [Abstract] [Full Text] [PDF]

				A. C. Schmidt, J. M. McAuliffe, A. Huang, S. R. Surman, J. E. Bailly, W. R. Elkins, P. L. Collins, B. R. Murphy, and M. H. Skiadopoulos Bovine Parainfluenza Virus Type 3 (BPIV3) Fusion and Hemagglutinin-Neuraminidase Glycoproteins Make an Important Contribution to the Restricted Replication of BPIV3 in Primates J. Virol., October 1, 2000; 74(19): 8922 - 8929. [Abstract] [Full Text]

				J. E. Bailly, J. M. McAuliffe, A. P. Durbin, W. R. Elkins, P. L. Collins, and B. R. Murphy A Recombinant Human Parainfluenza Virus Type 3 (PIV3) in Which the Nucleocapsid N Protein Has Been Replaced by That of Bovine PIV3 Is Attenuated in Primates J. Virol., April 1, 2000; 74(7): 3188 - 3195. [Abstract] [Full Text]