HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bernard, J. Articles by de Kinkelin, P. Search for Related Content PubMed PubMed Citation Articles by Bernard, J. Articles by de Kinkelin, P. Agricola Articles by Bernard, J. Articles by de Kinkelin, P.

Cloning and Sequencing the Messenger RNA of the N Gene of Viral Haemorrhagic Septicaemia Virus

¹ Laboratoire d'Ichtyopathologie, Centre de Recherches de Jouy en Josas, Domaine de Vilvert, 78350 Jouy en Josas, France,
² Eurogentec S.A., Campus du Sart Tilman, 4000 - Liège
and³ Laboratoire Central de Génie Génétique, Université de Liège, Liège, Belgium

The mRNA transcribed from the N gene of viral haemorrhagic septicaemia virus (VHSV) of salmonids has been cloned in Escherichia coli and expressed. Fusion proteins were recognized by monoclonal antibody directed against the N protein from the viral particle. A 1212 bp long open reading frame (ORF) coding for 404 amino acids with a calculated M_r of 44590 was deduced from the nucleotide sequence. The ORF was preceded by a 93 bp segment including in position 42 the AACAC pentanucleotide which is presumed to be the start signal for transcription by analogy with other rhabdoviral mRNAs. The upstream 41 bp region could correspond to the covalently linked positive polarity leader RNA as also found on the N mRNA from infectious haematopoietic necrosis virus (IHNV). This may be a characteristic of fish lyssaviruses. The AAACC sequence, which is part of the leader, was not found. Amino acids 44 to 359 from IHNV and 45 to 360 from VHSV are 45.3% homologous. A strong homology which could reflect functional importance was also found for potential phosphorylation sites and hydrophobic peaks despite the fact that the two viruses evolved on different continents.

Received 30 October 1989; accepted 9 April 1990.

This article has been cited by other articles:

				P. Boudinot, D. Bernard, S. Boubekeur, M.-I. Thoulouze, M. Bremont, and A. Benmansour The glycoprotein of a fish rhabdovirus profiles the virus-specific T-cell repertoire in rainbow trout J. Gen. Virol., October 1, 2004; 85(10): 3099 - 3108. [Abstract] [Full Text] [PDF]

				C. O'Farrell, N. Vaghefi, M. Cantonnet, B. Buteau, P. Boudinot, and A. Benmansour Survey of Transcript Expression in Rainbow Trout Leukocytes Reveals a Major Contribution of Interferon-Responsive Genes in the Early Response to a Rhabdovirus Infection J. Virol., August 15, 2002; 76(16): 8040 - 8049. [Abstract] [Full Text] [PDF]

				P. Boudinot, S. Boubekeur, and A. Benmansour Rhabdovirus Infection Induces Public and Private T Cell Responses in Teleost Fish J. Immunol., December 1, 2001; 167(11): 6202 - 6209. [Abstract] [Full Text] [PDF]

				Y Gaudin, P de Kinkelin, and A Benmansour Mutations in the glycoprotein of viral haemorrhagic septicaemia virus that affect virulence for fish and the pH threshold for membrane fusion J. Gen. Virol., May 1, 1999; 80(5): 1221 - 1229. [Abstract]

				P. Boudinot, P. Massin, M. Blanco, S. Riffault, and A. Benmansour vig-1, a New Fish Gene Induced by the Rhabdovirus Glycoprotein, Has a Virus-Induced Homologue in Humans and Shares Conserved Motifs with the MoaA Family J. Virol., March 1, 1999; 73(3): 1846 - 1852. [Abstract] [Full Text] [PDF]

				T Said, H Bruley, A Lamoureux, and M Bremont An RNA-binding domain in the viral haemorrhagic septicaemia virus nucleoprotein J. Gen. Virol., January 1, 1998; 79(1): 47 - 50. [Abstract]