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J Gen Virol 71 (1990), 1835-1838; DOI 10.1099/0022-1317-71-8-1835
© 1990 Society for General Microbiology
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Production of Infectious Swine Vesicular Disease Virus from Cloned cDNA in Mammalian Cells

Toru Inoue1, Shigeo Yamaguchi1, Takakiyo Saeki1 and Kiichi Sekiguchi2

1 Exotic Disease Research Division, National Institute of Animal Health, Jyousuihonchyo, Kodaira-shi, Tokyo 187
and2 Epizootic Research Station, Equine Research Institute, Kokubunji-machi, Shimotsuga-gun, Tochigi-ken 329-04, Japan

Full-length cDNA clones of the swine vesicular disease virus (SVDV) were constructed from subgenomic cDNA clones in the expression vector pSVL (pSVLS00). The direct transfection of mammalian cells with plasmid pSVLS00 results in the production of infectious virus. The recovered virus was neutralized completely by anti-SVDV guinea-pig serum, but did show a difference in plaque morphology from the parental virus.

Received 14 March 1990; accepted 9 May 1990.


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