HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Marshall, J. J. A. Articles by Roy, P. Search for Related Content PubMed PubMed Citation Articles by Marshall, J. J. A. Articles by Roy, P. Agricola Articles by Marshall, J. J. A. Articles by Roy, P.

Biophysical Studies on the Morphology of Baculovirus-expressed Bluetongue Virus Tubules

¹ NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, U.K.
and² University of Alabama at Birmingham, School of Public Health, Department of Environmental Health Sciences, University Station, Birmingham, Alabama 35294, U.S.A.

Bluetongue virus tubules were purified from Spodoptera frugiperda cells infected with a recombinant baculovirus containing the NS1 gene from bluetongue virus serotype 10, and expressed under control of the Autographa californica nuclear polyhedrosis virus polyhedrin promoter. These tubules were subjected to a variety of chemical and physical treatments and the resulting effects on tubule morphology were examined by electron microscopy. A number of morphological similarities were noted between bluetongue virus tubules and cellular microtubules despite a lack of homology between the component proteins at the primary sequence level. A possible multistranded helical configuration is proposed for the tubule structure.

Received 16 January 1990; accepted 27 March 1990.