HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Takahashi, K. Articles by Nakanishi, S. Search for Related Content PubMed PubMed Citation Articles by Takahashi, K. Articles by Nakanishi, S. Agricola Articles by Takahashi, K. Articles by Nakanishi, S.

Cloning, sequencing and expression in Escherichia coli of cDNA for a non-A, non-B hepatitis-associated microtubular aggregates protein

Naomi Kitamura^2,

¹ Biosciences Laboratory, Life Science Research Sector, Research Center, Mitsubishi Kasei Corporation, 1000 Kamoshida-cho, Midori-ku, Yokohama 227
² Institute for Immunology, Kyoto University, Faculty of Medicine, Yoshida, Sakyo-ku, Kyoto 606
and³ Department of Enteroviruses, National Institute of Health, Musashimurayama, Tokyo 190-12, Japan

A 1.7 kb cDNA encoding a novel antigen (p44; apparent M_r 44K) associated with non-A, non-B (NANB) hepatitis, was isolated from the hepatic cDNA library of a chimpanzee infected with NANB hepatitis. The library was screened with a monoclonal antibody against this antigen. The cDNA cloned contained an open reading frame encoding a 444 amino acid protein with an M_r calculated to be 50468. The cDNA hybridized to a 1.9 kb mRNA obtained from chimpanzee hepatocytes infected with either the NANB or hepatitis delta viruses. It hybridized weakly to mRNA from hepatitis B virus-infected hepatocytes, and not at all to mRNA from normal chimpanzee hepatocytes. Southern blot analysis revealed that p44 is a host protein in chimpanzees, and that an identical gene exists in the human genome.

Present address: Institute for Liver Research, Kansai Medical School, 1 Fumizono-cho, Moriguchi, Osaka 570, Japan.

Received 1 February 1990; accepted 9 May 1990.

This article has been cited by other articles:

				K. L. Carter, E. Cahir-McFarland, and E. Kieff Epstein-Barr Virus-Induced Changes in B-Lymphocyte Gene Expression J. Virol., September 11, 2002; 76(20): 10427 - 10436. [Abstract] [Full Text] [PDF]