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Mapping of antigenic sites on the bovine ephemeral fever virus glycoprotein using monoclonal antibodies

CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Private Bag No 3 PO, Indooroopilly, Queensland 4086, Australia

Monoclonal antibodies (MAbs) were produced against the G, M2 and N proteins of bovine ephemeral fever virus (BEFV) and 29 were selected for further study. Thirteen neutralizing MAbs were assigned to one conformation-independent and at least two conformation-dependent antigenic sites on the G protein by a competitive binding ELISA. The panel of MAbs were tested by neutralization and immunofluorescence with three strains of BEFV and three BEFV-related viruses. The results indicated that BEFV strains from different sources were not identical and that the M2 protein was the least variable of the proteins investigated. Passive protection studies in mice showed that the correlation between neutralizing titre and resistance to challenge was 0.85 (P < 0.001).

Received 9 January 1990; accepted 9 May 1990.

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