| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, U.K.
and2 Department of Environmental Health Sciences, School of Public Health 408th, The University of Alabama at Birmingham, University Station, Birmingham, Alabama 35294, U.S.A.
A DNA clone of RNA segment 8 (S8) of bluetongue virus type 10 (BTV-10), an orbivirus member of the Reoviridae family has been expressed to high levels (20 mg/1x109 cells) using an Autographa californica nuclear polyhedrosis virus expression vector (pA-cYM1). The expressed protein is similar to the authentic BTV phosphoprotein NS2, in its size, antigenicity, and also the manner of phosphorylation (e.g. same peptides and residues). Both mammalian and insect cell-derived NS2 proteins are phosphorylated at serine residues only. Using affinity column chromatography and a gel retardation assay, the expressed protein has been shown to possess ssRNA-binding ability, a property which is shown to be independent of the phosphorylation state of the protein. In immunoelectron micrographic studies, gold-labelled anti-expressed NS2 antibodies have been used to localize the NS2 protein within the viral inclusion bodies (VIBs) in BTV-infected mammalian cells. Large inclusion bodies, morphologically similar to VIBs, have been identified in the recombinant virus-infected Spodoptera frugiperda cells. These structures have been shown to react with gold-labelled anti-BTV-10 antisera, demonstrating the first direct evidence of the origin of inclusion bodies in orbivirus infection.
Received 7 March 1990;
accepted 24 May 1990.
This article has been cited by other articles:
|
|
 |
|
  P. Roy Bluetongue virus: dissection of the polymerase complex J. Gen. Virol., August 1, 2008; 89(8): 1789 - 1804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sen, N. Sen, and E. R. Mackow The Formation of Viroplasm-Like Structures by the Rotavirus NSP5 Protein Is Calcium Regulated and Directed by a C-Terminal Helical Domain J. Virol., November 1, 2007; 81(21): 11758 - 11767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Miller, M. M. Arnold, T. J. Broering, C. Eichwald, J. Kim, J. B. Dinoso, and M. L. Nibert Virus-derived Platforms for Visualizing Protein Associations inside Cells Mol. Cell. Proteomics, June 1, 2007; 6(6): 1027 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wei, T. Shimizu, K. Hagiwara, A. Kikuchi, Y. Moriyasu, N. Suzuki, H. Chen, and T. Omura Pns12 protein of Rice dwarf virus is essential for formation of viroplasms and nucleation of viral-assembly complexes J. Gen. Virol., February 1, 2006; 87(2): 429 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Kar, N. Iwatani, and P. Roy Assembly and Intracellular Localization of the Bluetongue Virus Core Protein VP3 J. Virol., September 1, 2005; 79(17): 11487 - 11495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Modrof, K. Lymperopoulos, and P. Roy Phosphorylation of Bluetongue Virus Nonstructural Protein 2 Is Essential for Formation of Viral Inclusion Bodies J. Virol., August 1, 2005; 79(15): 10023 - 10031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Butan, H. van der Zandt, and P. A. Tucker Structure and Assembly of the RNA Binding Domain of Bluetongue Virus Non-structural Protein 2 J. Biol. Chem., September 3, 2004; 279(36): 37613 - 37621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Owens, C. Limn, and P. Roy Role of an Arbovirus Nonstructural Protein in Cellular Pathogenesis and Virus Release J. Virol., June 15, 2004; 78(12): 6649 - 6656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lymperopoulos, C. Wirblich, I. Brierley, and P. Roy Sequence Specificity in the Interaction of Bluetongue Virus Non-structural Protein 2 (NS2) with Viral RNA J. Biol. Chem., August 22, 2003; 278(34): 31722 - 31730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Broering, J. S. L. Parker, P. L. Joyce, J. Kim, and M. L. Nibert Mammalian Reovirus Nonstructural Protein {micro}NS Forms Large Inclusions and Colocalizes with Reovirus Microtubule-Associated Protein {micro}2 in Transfected Cells J. Virol., July 17, 2002; 76(16): 8285 - 8297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. F. Taraporewala and J. T. Patton Identification and Characterization of the Helix-Destabilizing Activity of Rotavirus Nonstructural Protein NSP2 J. Virol., May 15, 2001; 75(10): 4519 - 4527. [Abstract] [Full Text]
|
|
|
|
|
|
N. J. Horscroft and P. Roy NTP binding and phosphohydrolase activity associated with purified bluetongue virus non-structural protein NS2 J. Gen. Virol., August 1, 2000; 81(8): 1961 - 1965. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Taraporewala, D. Chen, and J. T. Patton Multimers Formed by the Rotavirus Nonstructural Protein NSP2 Bind to RNA and Have Nucleoside Triphosphatase Activity J. Virol., December 1, 1999; 73(12): 9934 - 9943. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
