| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Yale Arbovirus Research Unit, Yale University School of Medicine, New Haven, Connecticut 06510
and2 Department of Biochemistry and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts 01003, U.S.A.
The antigenic structures of the envelope protein, E, and the non-structural protein, NS1, of dengue type 1 virus (DEN1) have been studied in the form of recombinant fusion proteins expressed in Escherichia coli. Deletion analysis was used to identify two distinct antigenic domains in E that reacted with subsets of antiviral monoclonal antibodies (MAbs). Domain I of E extends from amino acid residues (aa) 76 to 93 of E; domain II extends from aa 293 to 402 and contains an essential disulphide bridge. MAbs also reacted with several determinants clustered near the N terminus of the NS1 protein (aa 57 to 126). Recombinant fusion proteins containing E. coli trpE sequences and most of the sequences for either E or NS1 were immunogenic in mice. The antibodies elicited by the E fusion protein reacted with a portion of the protein containing domain II, whereas antibodies elicited by the NS1 fusion protein did not react with the antigenic determinants defined by our MAbs.
Received 26 January 1990;
accepted 15 May 1990.
This article has been cited by other articles:
|
|
 |
|
  S. Sukupolvi-Petty, S. K. Austin, W. E. Purtha, T. Oliphant, G. E. Nybakken, J. J. Schlesinger, J. T. Roehrig, G. D. Gromowski, A. D. Barrett, D. H. Fremont, et al. Type- and Subcomplex-Specific Neutralizing Antibodies against Domain III of Dengue Virus Type 2 Envelope Protein Recognize Adjacent Epitopes J. Virol., December 1, 2007; 81(23): 12816 - 12826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Schepp-Berglind, M. Luo, D. Wang, J. A. Wicker, N. U. Raja, B. D. Hoel, D. H. Holman, A. D. T. Barrett, and J. Y. Dong Complex Adenovirus-Mediated Expression of West Nile Virus C, PreM, E, and NS1 Proteins Induces both Humoral and Cellular Immune Responses Clin. Vaccine Immunol., September 1, 2007; 14(9): 1117 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Chen, H.-N. Huang, C.-T. Lin, Y.-F. Chen, C.-C. King, and H.-C. Wu Generation and Characterization of Monoclonal Antibodies against Dengue Virus Type 1 for Epitope Mapping and Serological Detection by Epitope-Based Peptide Antigens Clin. Vaccine Immunol., April 1, 2007; 14(4): 404 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. B. D. SANTOS, M. P. MIAGOSTOVICH, R. M. R. NOGUEIRA, H. G. SCHATZMAYR, L. W. RILEY, and E. HARRIS ANALYSIS OF RECOMBINANT DENGUE VIRUS POLYPEPTIDES FOR DENGUE DIAGNOSIS AND EVALUATION OF THE HUMORAL IMMUNE RESPONSE Am J Trop Med Hyg, August 1, 2004; 71(2): 144 - 152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Roehrig, K. E. Volpe, J. Squires, A. R. Hunt, B. S. Davis, and G.-J. J. Chang Contribution of Disulfide Bridging to Epitope Expression of the Dengue Type 2 Virus Envelope Glycoprotein J. Virol., March 1, 2004; 78(5): 2648 - 2652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-C. Wu, M.-Y. Jung, C.-Y. Chiu, T.-T. Chao, S.-C. Lai, J.-T. Jan, and M.-F. Shaio Identification of a dengue virus type 2 (DEN-2) serotype-specific B-cell epitope and detection of DEN-2-immunized animal serum samples using an epitope-based peptide antigen J. Gen. Virol., October 1, 2003; 84(10): 2771 - 2779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Cuzzubbo, T. P. Endy, A. Nisalak, S. Kalayanarooj, D. W. Vaughn, S. A. Ogata, D. E. Clements, and P. L. Devine Use of Recombinant Envelope Proteins for Serological Diagnosis of Dengue Virus Infection in an Immunochromatographic Assay Clin. Vaccine Immunol., November 1, 2001; 8(6): 1150 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-L. Kao, M.-C. Wu, Y.-H. Chiu, J.-L. Lin, Y.-C. Wu, Y.-Y. Yueh, L.-K. Chen, M.-F. Shaio, and C.-C. King Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture J. Clin. Microbiol., October 1, 2001; 39(10): 3672 - 3677. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
