HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Oxford, J. S. Articles by Schild, G. C. Search for Related Content PubMed PubMed Citation Articles by Oxford, J. S. Articles by Schild, G. C. Agricola Articles by Oxford, J. S. Articles by Schild, G. C.

Direct Isolation in Eggs of Influenza A (H1N1) and B Viruses with Haemagglutinins of Different Antigenic and Amino Acid Composition

¹ Department of Medical Microbiology, The London Hospital Medical College, Whitechapel, London E1 2AD
and The² National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Hertfordshire EN6 3QG, U.K.

Influenza A (H1N1) and influenza B viruses from clinical samples were isolated in the amniotic cavity of embryonated hens' eggs by classical techniques and propagated in the allantoic cavity. Virus progeny from different eggs which had been inoculated with virus material from the same clinical sample possessed antigenically distinguishable haemagglutinins (HAs). Virus progeny of some eggs possessed HAs which were serologically identical to those of virus isolated in parallel in mammalian (MDCK) cells. These egg-grown viruses possessing HAs with the antigenic phenotype of mammalian cell-grown viruses appeared to be antigenically related to epidemic influenza virus because post-infection human sera reacted to high titre with the virus HA. Specific nucleotide changes were detected in the HAs of the viruses isolated directly in eggs at positions 163 and 189 for influenza A (H1Na) viruses or positions 141 and 196 to 198 for influenza B viruses. Egg-isolated viruses which possessed the antigenic phenotype of mammalian cell-grown viruses retained glycosylation sites at positions 163 and 196. The viruses isolated directly in embryonated hens' eggs which possessed the HA antigenic phenotype and glycosylation sites of MDCK cell-grown virus can, unlike the latter viruses themselves, be used as candidate influenza vaccine viruses.

Received 6 July 1990; accepted 4 October 1990.

This article has been cited by other articles:

				Q. Wang, F. Cheng, M. Lu, X. Tian, and J. Ma Crystal Structure of Unliganded Influenza B Virus Hemagglutinin J. Virol., March 15, 2008; 82(6): 3011 - 3020. [Abstract] [Full Text] [PDF]