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Nucleotide sequence and gene organization of the 3'-terminal region of chrysanthemum virus B genomic RNA

All-Union Institute of Agricultural Biotechnology, Moscow 127253, U.S.S.R.

DNA clones complementary to the 3'-terminal 3426 nucleotides of the genomic RNA of the carlavirus chrysanthemum virus B (CVB) have been sequenced. The sequence contains six open reading frames (ORFs) which encode putative proteins (in the 5' 3' direction) of M_r 25749 (ORF2), M_r 11435 (ORF3), M_r 6984 (ORF4), the triple gene block proteins, a protein M_r 34638 (ORF5); the coat protein, and a protein of M_r 12609 (ORF6). The latter protein is basic and contains a putative zinc finger motif. The 5'-proximal ORF1 encodes a product with substantial homology to the C-terminal portions of the putative RNA replicases of two other carlaviruses, potato viruses M and S. The analysis of the minus-sense sequence shows one ORF which encodes a polypeptide of M_r 16817. The sequenced portion of the CVB genomic RNA contains three short internal non-coding regions, two of which are typical for carlaviruses (those between ORFs 1 and 2, and between ORFs 4 and 5), and one (between ORFs 5 and 6) is unusual. There is significant similarity in the amino acid sequences of the CVB RNA-encoded proteins and the corresponding proteins of other carlaviruses.

Received 18 April 1991; accepted 3 July 1991.

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