| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Division of Vector-Borne Infectious Diseases, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, US Department of Health and Human Services, P.O. Box 2087
and2 Arthropod-Borne Infectious Diseases Laboratory, Colorado State University, C.S.U. Foothills Campus, Fort Collins, Colorado 80522, U.S.A.
Four monoclonal antibody-resistant variants (MARVs) of Venezuelan equine encephalitis (VEE) virus were used to study mosquito-virus interactions. In vitro experiments using an Aedes albopictus cell line, C6/36, demonstrated that an amino acid change in the glycoprotein E2h epitope (MARV 1A3B-7) decreased virus growth when compared with the wild-type, Trinidad donkey virus, and its vaccine derivative, TC-83. The MARVs replicated as efficiently as the parent virus when inoculated into Aedes aegypti mosquitoes, but MARV 1A3B-7 was restricted in its ability to infect and disseminate from the midgut following oral infection. These results demonstrate that a single amino acid change in the E2 glycoprotein can affect the ability of VEE virus to replicate and disseminate in Ae. aegypti mosquitoes.
Received 5 February 1991;
accepted 22 May 1991.
This article has been cited by other articles:
|
|
 |
|
  D. J. Pierro, E. L. Powers, and K. E. Olson Genetic Determinants of Sindbis Virus Mosquito Infection Are Associated with a Highly Conserved Alphavirus and Flavivirus Envelope Sequence J. Virol., March 15, 2008; 82(6): 2966 - 2974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Pierro, E. L. Powers, and K. E. Olson Genetic determinants of Sindbis virus strain TR339 affecting midgut infection in the mosquito Aedes aegypti J. Gen. Virol., May 1, 2007; 88(5): 1545 - 1554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. McELROY, K. A. TSETSARKIN, D. L. VANLANDINGHAM, and S. HIGGS MANIPULATION OF THE YELLOW FEVER VIRUS NON-STRUCTURAL GENES 2A AND 4B AND THE 3'NON-CODING REGION TO EVALUATE GENETIC DETERMINANTS OF VIRAL DISSEMINATION FROM THE AEDES AEGYPTI MIDGUT Am J Trop Med Hyg, December 1, 2006; 75(6): 1158 - 1164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. McElroy, K. A. Tsetsarkin, D. L. Vanlandingham, and S. Higgs Role of the yellow fever virus structural protein genes in viral dissemination from the Aedes aegypti mosquito midgut. J. Gen. Virol., October 1, 2006; 87(Pt 10): 2993 - 3001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. VANLANDINGHAM, K. TSETSARKIN, K. A. KLINGLER, C. HONG, K. L. MCELROY, M. J. LEHANE, and S. HIGGS DETERMINANTS OF VECTOR SPECIFICITY OF O'NYONG NYONG AND CHIKUNGUNYA VIRUSES IN ANOPHELES AND AEDES MOSQUITOES Am J Trop Med Hyg, April 1, 2006; 74(4): 663 - 669. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Brault, A. M. Powers, D. Ortiz, J. G. Estrada-Franco, R. Navarro-Lopez, and S. C. Weaver Venezuelan equine encephalitis emergence: Enhanced vector infection from a single amino acid substitution in the envelope glycoprotein PNAS, August 3, 2004; 101(31): 11344 - 11349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Myles, D. J. Pierro, and K. E. Olson Deletions in the Putative Cell Receptor-Binding Domain of Sindbis Virus Strain MRE16 E2 Glycoprotein Reduce Midgut Infectivity in Aedes aegypti J. Virol., August 15, 2003; 77(16): 8872 - 8881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. SAHU, D. D. PEDERSEN, A. L. JENNY, B. J. SCHMITT, and A. D. ALSTAD PATHOGENICITY OF A VENEZUELAN EQUINE ENCEPHALOMYELITIS SEROTYPE IE VIRUS ISOLATE FOR PONIES Am J Trop Med Hyg, April 1, 2003; 68(4): 485 - 494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Brault, A. M. Powers, and S. C. Weaver Vector Infection Determinants of Venezuelan Equine Encephalitis Virus Reside within the E2 Envelope Glycoprotein J. Virol., May 15, 2002; 76(12): 6387 - 6392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Powers, A. C. Brault, R. M. Kinney, and S. C. Weaver The Use of Chimeric Venezuelan Equine Encephalitis Viruses as an Approach for the Molecular Identification of Natural Virulence Determinants J. Virol., May 1, 2000; 74(9): 4258 - 4263. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
