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J Gen Virol 72 (1991), 2475-2481; DOI 10.1099/0022-1317-72-10-2475
© 1991 Society for General Microbiology
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Antigenic structure of chimeras of type 1 and type 3 polioviruses involving antigenic sites 2, 3 and 4

P. D. Minor1, M. Ferguson1, K. Katrak1, D. Wood1, A. John1, J. Howlett1, G. Dunn1, K. Burke2 and J. W. Almond2

1 National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG
and2 Department of Microbiology, University of Reading, London Road, Reading RG1 5AQ, U.K.

Chimeric polioviruses have been made in which regions of the type 1 Sabin strain corresponding to antigenic sites 2, 3 and 4 have been replaced by the corresponding regions of the type 3 Sabin strain. Manipulation of one site or a component of it generally did not affect the reactions of the others, suggesting that they form independent structural features. The extent to which the inserted site expressed the antigenic properties of type 3 could be assessed by reaction with polyclonal or monoclonal antibodies, or by immunogenicity. Site 2 could be expressed on infectious virus and site 3 on heated non-infectious virus (C antigen), but not on the native virion. The results are consistent with the view that sites consisting of a continuous sequence of amino acids may be presented on chimeras, whereas more complex sites, such as site 4 or site 3 of the native virion, are transferred less readily from type 3 to type 1.

Received 8 May 1991; accepted 28 June 1991.


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Copyright © 1991 by the Society for General Microbiology.