| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Pathology
and2 AIDS Research Center, National Institute of Health, 2-10-35, Kamiosaki, Shinagawa-ku, Tokyo 141
3 Biological Science Laboratory, Nippon Zeon Company Ltd, Yako, Kawasaki-ku, Kawasaki-shi, Kanagawa,
and4 Basic Research Laboratories, Toray Industries Inc., Tebiro, Kamakura, Kanagawa, Japan
An ultrastructural study was performed on rabbit epithelial RK-13 cells and CD4+ human T lymphocyte lines infected with various recombinant vaccinia viruses (RVVs) expressing genes of human immunodeficiency virus (HIV): the mature p17 or p24 gag domain alone, the entire or truncated gag gene, the reverse transcriptase domain, or the gag-pol genes with a frameshift mutation. Cells infected with RVVs that produced the gag polyprotein with a predicted Mr of more than 48K showed budding and release of HIV-like particles into the extracellular space. These particles were not observed in cells expressing a truncated gag gene (p17 and p24 regions). Mature HIV-like particles were observed extracellularly when the entire gag gene and the protease region of the pol gene were expressed. In contrast, in cells infected with RVVs that contained the gag-pol gene with a frameshift mutation, neither recognizable budding structures nor extracellular HIV-like particles could be detected. These results suggest that the gag gene, particularly its 3' terminus, is necessary for the assembly of HIV particles. In addition, the protease region of the pol gene seems to be required for morphological maturation of HIV particles, but complete proteolytic cleavage of the gag protein may prevent bud formation.
Received 4 March 1991;
accepted 19 June 1991.
This article has been cited by other articles:
|
|
 |
|
  I. B. Hogue, A. Hoppe, and A. Ono Quantitative Fluorescence Resonance Energy Transfer Microscopy Analysis of the Human Immunodeficiency Virus Type 1 Gag-Gag Interaction: Relative Contributions of the CA and NC Domains and Membrane Binding J. Virol., July 15, 2009; 83(14): 7322 - 7336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Morikawa, T. Goto, D. Yasuoka, F. Momose, and T. Matano Defect of Human Immunodeficiency Virus Type 2 Gag Assembly in Saccharomyces cerevisiae J. Virol., September 15, 2007; 81(18): 9911 - 9921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wirblich, B. Bhattacharya, and P. Roy Nonstructural Protein 3 of Bluetongue Virus Assists Virus Release by Recruiting ESCRT-I Protein Tsg101 J. Virol., January 1, 2006; 80(1): 460 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ono, A. A. Waheed, A. Joshi, and E. O. Freed Association of Human Immunodeficiency Virus Type 1 Gag with Membrane Does Not Require Highly Basic Sequences in the Nucleocapsid: Use of a Novel Gag Multimerization Assay J. Virol., November 15, 2005; 79(22): 14131 - 14140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Morikawa, T. Goto, and F. Momose Human Immunodeficiency Virus Type 1 Gag Assembly through Assembly Intermediates J. Biol. Chem., July 23, 2004; 279(30): 31964 - 31972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yokomaku, H. Miura, H. Tomiyama, A. Kawana-Tachikawa, M. Takiguchi, A. Kojima, Y. Nagai, A. Iwamoto, Z. Matsuda, and K. Ariyoshi Impaired Processing and Presentation of Cytotoxic-T-Lymphocyte (CTL) Epitopes Are Major Escape Mechanisms from CTL Immune Pressure in Human Immunodeficiency Virus Type 1 Infection J. Virol., February 1, 2004; 78(3): 1324 - 1332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shoya, K. Tokunaga, H. Sawa, M. Maeda, T. Ueno, T. Yoshikawa, H. Hasegawa, T. Sata, T. Kurata, W. W. Hall, et al. Human topoisomerase I promotes HIV-1 proviral DNA synthesis: Implications for the species specificity and cellular tropism of HIV-1 infection PNAS, July 8, 2003; 100(14): 8442 - 8447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. O. Freed Viral Late Domains J. Virol., April 16, 2002; 76(10): 4679 - 4687. [Full Text] [PDF]
|
|
|
|
|
|
D. G. Demirov, J. M. Orenstein, and E. O. Freed The Late Domain of Human Immunodeficiency Virus Type 1 p6 Promotes Virus Release in a Cell Type-Dependent Manner J. Virol., January 1, 2002; 76(1): 105 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ohba, T. Soga, T. Tomozawa, Y. Nishikawa, A. Yasuda, A. Kojima, T. Kurata, and J. Chiba An immunodominant neutralization epitope on the 'thumb' subdomain of human immunodeficiency virus type 1 reverse transcriptase revealed by phage display antibodies J. Gen. Virol., April 1, 2001; 82(4): 813 - 820. [Abstract] [Full Text]
|
|
|
|
|
|
S. Sandefur, R. M. Smith, V. Varthakavi, and P. Spearman Mapping and Characterization of the N-Terminal I Domain of Human Immunodeficiency Virus Type 1 Pr55Gag J. Virol., August 15, 2000; 74(16): 7238 - 7249. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Accola, B. Strack, and H. G. Göttlinger Efficient Particle Production by Minimal Gag Constructs Which Retain the Carboxy-Terminal Domain of Human Immunodeficiency Virus Type 1 Capsid-p2 and a Late Assembly Domain J. Virol., June 15, 2000; 74(12): 5395 - 5402. [Abstract] [Full Text]
|
|
|
|
|
|
A. Ono, D. Demirov, and E. O. Freed Relationship between Human Immunodeficiency Virus Type 1 Gag Multimerization and Membrane Binding J. Virol., June 1, 2000; 74(11): 5142 - 5150. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Morikawa, D. J. Hockley, M. V. Nermut, and I. M. Jones Roles of Matrix, p2, and N-Terminal Myristoylation in Human Immunodeficiency Virus Type 1 Gag Assembly J. Virol., January 1, 2000; 74(1): 16 - 23. [Abstract] [Full Text]
|
|
|
|
|
|
S. D. Parker and E. Hunter A Cell-Line-Specific Defect in the Intracellular Transport and Release of Assembled Retroviral Capsids J. Virol., January 1, 2000; 74(2): 784 - 795. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Morikawa, T. Goto, and K. Sano In Vitro Assembly of Human Immunodeficiency Virus Type 1 Gag Protein J. Biol. Chem., September 24, 1999; 274(39): 27997 - 28002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Garnier, L. Ratner, B. Rovinski, S.-X. Cao, and J. W. Wills Particle Size Determinants in the Human Immunodeficiency Virus Type 1 Gag Protein J. Virol., June 1, 1998; 72(6): 4667 - 4677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gross, H. Hohenberg, C. Huckhagel, and H.-G. Krausslich N-Terminal Extension of Human Immunodeficiency Virus Capsid Protein Converts the In Vitro Assembly Phenotype from Tubular to Spherical Particles J. Virol., June 1, 1998; 72(6): 4798 - 4810. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
