HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Calvert, J. G. Articles by Dobos, P. Search for Related Content PubMed PubMed Citation Articles by Calvert, J. G. Articles by Dobos, P. Agricola Articles by Calvert, J. G. Articles by Dobos, P.

Characterization of the VPg-dsRNA linkage of infectious pancreatic necrosis virus

Jay G. Calvert

Eva Nagy^>

Maritza Soler

Department of Microbiology, College of Biological Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1

By the use of strong denaturing agents, a genome-linked protein (VPg)-RNA complex was purified from infectious pancreatic necrosis virus. Ribonuclease treatment of ¹²⁵I-labelled VPg-RNA released a 90K polypeptide identical to the minor structural polypeptide VP1 (the putative RNA polymerase), as determined by peptide mapping. The polypeptide is linked to the RNA by a serine-5' GMP phosphodiester bond. The results identify birnaviruses as the only dsRNA viruses with a VPg, the size of which is the largest of the VPgs of RNA viruses.

Present address: USDA, ARS Regional Poultry Research Laboratory, 3606 East Mountain Hope Road, East Lansing, Michigan 48823, U.S.A.

^> Present address: Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Ontario N1G 2W1, Canada.

Present address: Laboratorio de Bioquimica, Facultad de Medicina, Universidad de Valparaiso, Casilla 92-V, Valparaiso, Chile.

Received 3 April 1991; accepted 18 June 1991.

This article has been cited by other articles:

				T. Pedersen, A. Skjesol, and J. B. Jorgensen VP3, a Structural Protein of Infectious Pancreatic Necrosis Virus, Interacts with RNA-Dependent RNA Polymerase VP1 and with Double-Stranded RNA J. Virol., June 15, 2007; 81(12): 6652 - 6663. [Abstract] [Full Text] [PDF]

				J. Pan, V. N. Vakharia, and Y. J. Tao The structure of a birnavirus polymerase reveals a distinct active site topology PNAS, May 1, 2007; 104(18): 7385 - 7390. [Abstract] [Full Text] [PDF]

				H. Song, N. Santi, O. Evensen, and V. N. Vakharia Molecular Determinants of Infectious Pancreatic Necrosis Virus Virulence and Cell Culture Adaptation J. Virol., August 15, 2005; 79(16): 10289 - 10299. [Abstract] [Full Text] [PDF]

				R. A. Villanueva, J. L. Galaz, J. A. Valdes, M. M. Jashes, and A. M. Sandino Genome Assembly and Particle Maturation of the Birnavirus Infectious Pancreatic Necrosis Virus J. Virol., December 15, 2004; 78(24): 13829 - 13838. [Abstract] [Full Text] [PDF]

				H. J. Boot and S. B. E. Pritz-Verschuren Modifications of the 3'-UTR stem-loop of infectious bursal disease virus are allowed without influencing replication or virulence Nucleic Acids Res., January 12, 2004; 32(1): 211 - 222. [Abstract] [Full Text] [PDF]

				K. Yao and V. N. Vakharia Generation of Infectious Pancreatic Necrosis Virus from Cloned cDNA J. Virol., November 1, 1998; 72(11): 8913 - 8920. [Abstract] [Full Text] [PDF]