| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Immunology Division, Jichi Medical School, Minamikawachi-Machi, Tochigi-Ken 329-04
2 First Department of Internal Medicine, Yamanashi Medical College, Yamanashi-Ken 409-38
3 Department of Hygiene, University of Hiroshima, Hiroshima-Ken 734
4 Japanese Red Cross Blood Center, Saitama-Ken 362
5 Department of Immunology, the Kitasato Institute, Tokyo 108
and6 Mita Institute, Tokyo 108, Japan
The complete nucleotide sequence of a hepatitis C virus derived from plasma of a human carrier in Japan was determined. The cDNA of the isolate (HC-J6) contained 9481 nucleotides and an additional T stretch of 30 to 108 nucleotides at the 3' end, and had one large open reading frame coding for a polyprotein of 3033 amino acids. It differed by 31·8 to 32·1% in the nucleotide sequence and by 27·4 to 27·7% in the amino acid sequence from an American isolate and two Japanese isolates previously reported. Among these four isolates, the 5' non-coding region of 329 to 341 nucleotides was well conserved (>93% identity), whereas the 3' non-coding region of 39 to 45 nucleotides (T stretches not included) was more variable (>30% identity). An excellent degree of conservation of the 5' non-coding region would reflect its pivotal role in replication, and primers deduced from this region could be applied for the sensitive and specific detection of viral RNA by polymerase chain reaction. Due to a high degree of similarity in the amino acid sequence of the putative core protein (>90%), antigen probes deduced from it would be suitable for the serological diagnosis of HCV infection. Low sequence similarity in the putative envelope protein (>53% identity), however, would have to be taken into account in considering the immunoprophylaxis of HCV infection.
Received 20 May 1991;
accepted 29 July 1991.
This article has been cited by other articles:
|
|
 |
|
  H. Li, L. V. Thomassen, A. Majid, B. J. McMahon, D. Bruden, S. McArdle, N. Bano, M. Chung, R. L. Carithers, J. D. Perkins, et al. Investigation of Putative Multisubtype Hepatitis C Virus Infections In Vivo by Heteroduplex Mobility Analysis of Core/Envelope Subgenomes J. Virol., August 1, 2008; 82(15): 7524 - 7532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zehender, C. De Maddalena, F. Bernini, E. Ebranati, G. Monti, P. Pioltelli, and M. Galli Compartmentalization of Hepatitis C Virus Quasispecies in Blood Mononuclear Cells of Patients with Mixed Cryoglobulinemic Syndrome J. Virol., July 15, 2005; 79(14): 9145 - 9156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ni, E. Hembrador, A. M. Di Bisceglie, I. M. Jacobson, A. H. Talal, D. Butera, C. M. Rice, T. J. Chambers, and L. B. Dustin Accumulation of B Lymphocytes with a Naive, Resting Phenotype in a Subset of Hepatitis C Patients J. Immunol., March 15, 2003; 170(6): 3429 - 3439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Corbet, J. Bukh, A. Heinsen, and A. Fomsgaard Hepatitis C Virus Subtyping by a Core-Envelope 1-Based Reverse Transcriptase PCR Assay with Sequencing and Its Use in Determining Subtype Distribution among Danish Patients J. Clin. Microbiol., March 1, 2003; 41(3): 1091 - 1100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Carmona and M. Molina Binding of oligonucleotides to a viral hairpin forming RNA triplexes with parallel G*G*C triplets Nucleic Acids Res., March 15, 2002; 30(6): 1333 - 1337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Lusida, M. Nagano-Fujii, C. A. Nidom, Soetjipto, R. Handajani, T. Fujita, K. Oka, and H. Hotta Correlation between Mutations in the Interferon Sensitivity-Determining Region of NS5A Protein and Viral Load of Hepatitis C Virus Subtypes 1b, 1c, and 2a J. Clin. Microbiol., November 1, 2001; 39(11): 3858 - 3864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rho, S. Choi, Y. R. Seong, J. Choi, and D.-S. Im The Arginine-1493 Residue in QRRGRTGR1493G Motif IV of the Hepatitis C Virus NS3 Helicase Domain Is Essential for NS3 Protein Methylation by the Protein Arginine Methyltransferase 1 J. Virol., September 1, 2001; 75(17): 8031 - 8044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lu, T. Nakano, E. Orito, M. Mizokami, and B. H. Robertson Evaluation of Accumulation of Hepatitis C Virus Mutations in a Chronically Infected Chimpanzee: Comparison of the Core, E1, HVR1, and NS5b Regions J. Virol., March 15, 2001; 75(6): 3004 - 3009. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. N. Zein Clinical Significance of Hepatitis C Virus Genotypes Clin. Microbiol. Rev., April 1, 2000; 13(2): 223 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Kolykhalov, K. Mihalik, S. M. Feinstone, and C. M. Rice Hepatitis C Virus-Encoded Enzymatic Activities and Conserved RNA Elements in the 3' Nontranslated Region Are Essential for Virus Replication In Vivo J. Virol., February 15, 2000; 74(4): 2046 - 2051. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. E. Reed and C. M. Rice Identification of the Major Phosphorylation Site of the Hepatitis C Virus H Strain NS5A Protein as Serine 2321 J. Biol. Chem., September 24, 1999; 274(39): 28011 - 28018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schröter, H.-H. Feucht, P. Schäfer, B. Zöllner, and R. Laufs Serological Determination of Hepatitis C Virus Subtypes 1a, 1b, 2a, 2b, 3a, and 4a by a Recombinant Immunoblot Assay J. Clin. Microbiol., August 1, 1999; 37(8): 2576 - 2580. [Abstract] [Full Text]
|
|
|
|
|
|
N. Mamiya and H. J. Worman Hepatitis C Virus Core Protein Binds to a DEAD Box RNA Helicase J. Biol. Chem., May 28, 1999; 274(22): 15751 - 15756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tang, A. J. Collier, and R. M. Elliott Alterations to both the Primary and Predicted Secondary Structure of Stem-Loop IIIc of the Hepatitis C Virus 1b 5' Untranslated Region (5'UTR) Lead to Mutants Severely Defective in Translation Which Cannot Be Complemented in trans by the Wild-Type 5'UTR Sequence J. Virol., March 1, 1999; 73(3): 2359 - 2364. [Abstract] [Full Text]
|
|
|
|
|
|
M. Shirai, T. Arichi, M. Chen, M. Nishioka, K. Ikeda, H. Takahashi, N. Enomoto, T. Saito, M. E. Major, T. Nakazawa, et al. T Cell Recognition of Hypervariable Region-1 from Hepatitis C Virus Envelope Protein with Multiple Class II MHC Molecules in Mice and Humans: Preferential Help for Induction of Antibodies to the Hypervariable Region J. Immunol., January 1, 1999; 162(1): 568 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Das, M. Ott, A. Yamane, W. Tsai, M. Gromeier, F. Lahser, S. Gupta, and A. Dasgupta A Small Yeast RNA Blocks Hepatitis C Virus Internal Ribosome Entry Site (HCV IRES)-Mediated Translation and Inhibits Replication of a Chimeric Poliovirus under Translational Control of the HCV IRES Element J. Virol., July 1, 1998; 72(7): 5638 - 5647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. H. Wong, D. D. Dudley, N. H. Afdhal, J. Dienstag, C. M. Rice, L. Wang, M. Houghton, B. D. Walker, and M. J. Koziel Liver-Derived CTL in Hepatitis C Virus Infection: Breadth and Specificity of Responses in a Cohort of Persons with Chronic Infection J. Immunol., February 1, 1998; 160(3): 1479 - 1488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Worman Molecular biological methods in diagnosis and treatment of liver diseases Clin. Chem., August 1, 1997; 43(8): 1476 - 1486. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
