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J Gen Virol 72 (1991), 2733-2737; DOI 10.1099/0022-1317-72-11-2733
© 1991 Society for General Microbiology
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Intragenic and intergenic recombination between temperature-sensitive mutants of vaccinia virus

Zahra Fathi{dagger}, Lyn M. Dyster, Janny Seto, Richard C. Condit{ddagger}> and Edward G. Niles

Department of Biochemistry, State University of New York at Buffalo, 140 Farber Hall, Buffalo, New York 14214, U.S.A.

The frequency of recombination for a complete set of two-factor crosses between vaccinia virus mutations separated by distances of between 54 and 10692 bp was determined. The results show that in intragenic crosses there is a linear relationship between the recombination frequency observed and distances between the mutations of up to 700 bp. However, no length dependence of the recombination frequency in intergenic crosses with a distance between mutations of 328 to greater than 10000 bp is observed. We attribute this lack of dependence to the high rate of viral DNA interchange, which makes some step other than the cross-over event rate-limiting. Furthermore, we believe that the observed difference in recombination frequency between inter- and intragenic recombination is due to complementation between temperature-sensitive mutants at the permissive temperature.

{dagger} Present address: Laboratory of Neurochemistry, NINDA, NIH, Building 36, Room 4D-20, 9000 Rockville Pike, Maryland 20892, U.S.A.

{ddagger}> Present address: Department of Immunology and Medical Microbiology, University of Florida, Gainesville, Florida 32610, U.S.A.

Received 24 April 1991; accepted 18 July 1991.




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Copyright © 1991 by the Society for General Microbiology.