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J Gen Virol 72 (1991), 2757-2764; DOI 10.1099/0022-1317-72-11-2757
© 1991 Society for General Microbiology
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Expression of HLA class I heavy chains and beta2-microglobulin does not affect human cytomegalovirus infectivity

M. F. C. Beersma1, P. M. E. Wertheim-van Dillen2, J. L. M. C. Geelen2 and T. E. W. Feltkamp1,3,

1 The Netherlands Ophthalmic Research Institute, P.O. Box 12141, 1100 AC Amsterdam
2 Department of Medical Microbiology, University of Amsterdam, Meibergdreef 15, 1100 AZ Amsterdam
and3 Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, P.O. Box 9190, 1006 AD Amsterdam, The Netherlands

Human cytomegalovirus (HCMV) purified from urine or tissue culture supernatant has been reported to contain beta2-microglobulin (beta2m), which forms the light chain of HLA class I molecules. It has been postulated that HCMV covered with beta2m binds to HLA class I {alpha}-chains at the cell surface. In the present study we used transfected human and mouse cell lines expressing distinct allelic forms of HLA class I and beta2m to determine whether HLA class I molecules could act as cellular receptors for HCMV. The susceptibility of cells to HCMV infection was estimated by calculating the percentage of cells expressing HCMV immediate early antigens. Although the results showed some variation between different transfected cell clones, no correlation was found between expression of HLA class I on the cell membrane and HCMV infection. Preincubation of HLA class I-positive cells with antibodies against HLA class I antigens inhibited HCMV infection after binding and adsorption of HCMV. Trypsin prevented HCMV infection of both class I-positive and class I-negative cells. We conclude that these results do not support the assumption that HLA class I molecules are functional receptors for HCMV.

Received 3 April 1991; accepted 6 August 1991.




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Copyright © 1991 by the Society for General Microbiology.