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J Gen Virol 72 (1991), 2923-2928; DOI 10.1099/0022-1317-72-12-2923
© 1991 Society for General Microbiology
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Bovine Immunodeficiency Virus: Immunochemical Characterization and Serological Survey

Marian Horzinek1, Lian Keldermans1, Thea Stuurman1, John Black3, Arnold Herrewegh1, Peter Sillekens2 and Marck Koolen2

1 Institute of Infectious Diseases and Immunology, Department of Virology, Veterinary Faculty, State University of Utrecht, Utrecht
2 Organon Teknika BV, Boxtel, The Netherlands
and3 American Bioresearch, Milton, Tennessee 37118, U.S.A.

Bovine immunodeficiency virus (BIV) was purified by isodensity centrifugation; viral activities were monitored in gradient fractions using the reverse transcriptase assay and a p26-specific monoclonal antibody ELISA. In the coincident peak fractions (density about 1.17 g/ml) proteins with Mr values of 26K, 17K, 53K, 14K and 100K (with decreasing intensity) were detected by Western blotting using serum of a calf after experimental BIV infection. When 957 randomly collected cattle sera from The Netherlands were tested by indirect immunofluorescence and confirmed using Western blot and/or radioimmunoprecipitation, 1.4% appeared seropositive. Thus BIV infection is not uncommon in one European cattle population.

Received 24 May 1991; accepted 21 August 1991.


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