HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by White, B. T. Articles by Elliott, R. M. Search for Related Content PubMed PubMed Citation Articles by White, B. T. Articles by Elliott, R. M. Agricola Articles by White, B. T. Articles by Elliott, R. M.

Further Characterization of Conditional Lethal Amber Nonsense Mutants of Vesicular Stomatitis Virus: Nucleotide Sequence Analysis

Brian T. White^1,2,

¹ MRC Virology Unit
and² Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, U.K.

Conditional lethal amber nonsense mutants of vesicular stomatitis virus, Indiana serotype, classified in complementation group I (the L gene), synthesize truncated versions of the L protein. This paper reports further characterization of mutants AmbL1, AmbL2 and AmbL3 by nucleic acid sequence analysis, which was achieved by sequencing L mRNA directly using appropriate synthetic oligonucleotides. In each case a single point mutation altered a glutamine-specifying codon to an amber stop codon. The L mRNA from wild-type and revertant viruses was sequenced for comparison. Of the revertants sequenced, each had reverted by back mutation within the same codon as the original mutation. A revertant of AmbL2 reverted by a second site mutation, also within the same codon as the original mutation. These mutants may be useful for assigning functions to different parts of the L polypeptide chain.

Present address: Inveresk Research International Ltd, Tranent EH33 2NE, U.K.

Received 21 June 1991; accepted 9 August 1991.