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Conversion of a Single-stranded Simian Virus 40 (SV40)-based Shuttle Vector to its Double-stranded Form does not Require the SV40 T antigen in Monkey Cells

Catherine Madzak

Laboratory of Molecular Genetics, Institut de Recherches Scientifiques sur le Cancer, BP no. 8, 94801 Villejuif, France

We have transfected the single-stranded DNA form of the simian virus 40 (SV40)-based shuttle vector pZ189 into CV1P simian cells. Although the strand used did not code for the T antigen, we observed its conversion to a double-stranded DNA form. We deduced that the replication step converting a circular single-stranded DNA to a double-stranded one is independent of the SV40 T antigen in simian cells.

Present address: Laboratory of Genetics, INRA Centre de Biotechnologies Agro-Industrielles, 78850 Thiverval-Grignon, France.

Received 28 May 1991; accepted 2 September 1991.