| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 7, Room 100, Bethesda, Maryland 20892, U.S.A.
The post-translational maturation of the fusion protein (F) of human respiratory syncytial virus was investigated. Chemical cross-linking experiments indicated that F forms homotetramers and provided evidence that the intermonomer contacts involve primarily the F1 subunit. Homooligomerization as measured by sedimentation in sucrose gradients was insensitive to carbonyl cyanide m-chlorophenylhydrazone, indicating that it occurs in the endoplasmic reticulum. Cleavage of the F0 precursor to yield the F1 and F2 subunits was blocked by monensin or brefeldin A, indicating that it takes place in distal cisternae of the trans Golgi compartment or in the more distal trans Golgi network. The F0 precursor was not detected at the cell surface in surface immunoprecipitation experiments, indicating that cleavage is intracellular. The appearance of the cleaved F1 protein at the cell surface was concurrent with that of the attachment glycoprotein (G); this and other information indicated that the type 2 membrane orientation of G is not obligatorily associated with a reduced transit rate. Examination of F maturation in the presence of tunicamycin provided evidence that its expression at the cell surface depends upon cleavage and not directly upon glycosylation.
Permanent address: Department of Microbiology, CMU, University of Geneva, Geneva, Switzerland.
Received 5 June 1991;
accepted 20 August 1991.
This article has been cited by other articles:
|
|
 |
|
  M. Shingai, M. Azuma, T. Ebihara, M. Sasai, K. Funami, M. Ayata, H. Ogura, H. Tsutsumi, M. Matsumoto, and T. Seya Soluble G protein of respiratory syncytial virus inhibits Toll-like receptor 3/4-mediated IFN-beta induction Int. Immunol., September 1, 2008; 20(9): 1169 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Liu, N. Bastien, and Y. Li Intracellular Processing, Glycosylation, and Cell Surface Expression of Human Metapneumovirus Attachment Glycoprotein J. Virol., December 15, 2007; 81(24): 13435 - 13443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Branigan, N. D. Day, C. Liu, L. L. Gutshall, J. A. Melero, R. T. Sarisky, and A. M. D. Vecchio The cytoplasmic domain of the F protein of Human respiratory syncytial virus is not required for cell fusion J. Gen. Virol., February 1, 2006; 87(2): 395 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Brock, J. M. Heck, P. A. McGraw, and J. E. Crowe Jr. The Transmembrane Domain of the Respiratory Syncytial Virus F Protein Is an Orientation-Independent Apical Plasma Membrane Sorting Sequence J. Virol., October 1, 2005; 79(19): 12528 - 12535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Schickli, J. Kaur, N. Ulbrandt, R. R. Spaete, and R. S. Tang An S101P Substitution in the Putative Cleavage Motif of the Human Metapneumovirus Fusion Protein Is a Major Determinant for Trypsin-Independent Growth in Vero Cells and Does Not Alter Tissue Tropism in Hamsters J. Virol., August 15, 2005; 79(16): 10678 - 10689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Gower, M. K. Pastey, M. E. Peeples, P. L. Collins, L. H. McCurdy, T. K. Hart, A. Guth, T. R. Johnson, and B. S. Graham RhoA Signaling Is Required for Respiratory Syncytial Virus-Induced Syncytium Formation and Filamentous Virion Morphology J. Virol., May 1, 2005; 79(9): 5326 - 5336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Pager, M. A. Wurth, and R. E. Dutch Subcellular Localization and Calcium and pH Requirements for Proteolytic Processing of the Hendra Virus Fusion Protein J. Virol., September 1, 2004; 78(17): 9154 - 9163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. McL. Rixon, G. Brown, J. Aitken, T. McDonald, S. Graham, and R. J. Sugrue The small hydrophobic (SH) protein accumulates within lipid-raft structures of the Golgi complex during respiratory syncytial virus infection J. Gen. Virol., May 1, 2004; 85(5): 1153 - 1165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. von Messling and R. Cattaneo N-Linked Glycans with Similar Location in the Fusion Protein Head Modulate Paramyxovirus Fusion J. Virol., October 1, 2003; 77(19): 10202 - 10212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Douglas, M. L. Panis, E. Ho, K.-Y. Lin, S. H. Krawczyk, D. M. Grant, R. Cai, S. Swaminathan, and T. Cihlar Inhibition of Respiratory Syncytial Virus Fusion by the Small Molecule VP-14637 via Specific Interactions with F Protein J. Virol., May 1, 2003; 77(9): 5054 - 5064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. McL. Rixon, C. Brown, G. Brown, and R. J. Sugrue Multiple glycosylated forms of the respiratory syncytial virus fusion protein are expressed in virus-infected cells J. Gen. Virol., January 1, 2002; 83(1): 61 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gonzalez-Reyes, M. B. Ruiz-Arguello, B. Garcia-Barreno, L. Calder, J. A. Lopez, J. P. Albar, J. J. Skehel, D. C. Wiley, and J. A. Melero Cleavage of the human respiratory syncytial virus fusion protein at two distinct sites is required for activation of membrane fusion PNAS, August 1, 2001; (2001) 151098198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Sugrue, C. Brown, G. Brown, J. Aitken, and H. W. McL. Rixon Furin cleavage of the respiratory syncytial virus fusion protein is not a requirement for its transport to the surface of virus-infected cells J. Gen. Virol., June 1, 2001; 82(6): 1375 - 1386. [Abstract] [Full Text]
|
|
|
|
|
|
G. Zimmer, I. Trotz, and G. Herrler N-Glycans of F Protein Differentially Affect Fusion Activity of Human Respiratory Syncytial Virus J. Virol., May 15, 2001; 75(10): 4744 - 4751. [Abstract] [Full Text]
|
|
|
|
|
|
A. Karger, U. Schmidt, and U. J. Buchholz Recombinant bovine respiratory syncytial virus with deletions of the G or SH genes: G and F proteins bind heparin J. Gen. Virol., March 1, 2001; 82(3): 631 - 640. [Abstract] [Full Text]
|
|
|
|
|
|
S. A. Feldman, S. Audet, and J. A. Beeler The Fusion Glycoprotein of Human Respiratory Syncytial Virus Facilitates Virus Attachment and Infectivity via an Interaction with Cellular Heparan Sulfate J. Virol., July 15, 2000; 74(14): 6442 - 6447. [Abstract] [Full Text]
|
|
|
|
|
|
M. K. Pastey, J. E. Crowe Jr., and B. S. Graham RhoA Interacts with the Fusion Glycoprotein of Respiratory Syncytial Virus and Facilitates Virus-Induced Syncytium Formation J. Virol., September 1, 1999; 73(9): 7262 - 7270. [Abstract] [Full Text]
|
|
|
|
|
|
H. Sakurai, R. A. Williamson, J. E. Crowe, J. A. Beeler, P. Poignard, R. B. Bastidas, R. M. Chanock, and D. R. Burton Human Antibody Responses to Mature and Immature Forms of Viral Envelope in Respiratory Syncytial Virus Infection: Significance for Subunit Vaccines J. Virol., April 1, 1999; 73(4): 2956 - 2962. [Abstract] [Full Text]
|
|
|
|
|
|
C. Bourgeois, J. B. Bour, K. Lidholt, C. Gauthray, and P. Pothier Heparin-Like Structures on Respiratory Syncytial Virus Are Involved in Its Infectivity In Vitro J. Virol., September 1, 1998; 72(9): 7221 - 7227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Lopez, R. Bustos, C. Orvell, M. Berois, J. Arbiza, B. Garcia-Barreno, and J. A. Melero Antigenic Structure of Human Respiratory Syncytial Virus Fusion Glycoprotein J. Virol., August 1, 1998; 72(8): 6922 - 6928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Li, T. Sergel, E. Razvi, and T. Morrison Effect of Cleavage Mutants on Syncytium Formation Directed by the Wild-Type Fusion Protein of Newcastle Disease Virus J. Virol., May 1, 1998; 72(5): 3789 - 3795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Zimmer, L. Budz, and G. Herrler Proteolytic Activation of Respiratory Syncytial Virus Fusion Protein. CLEAVAGE AT TWO FURIN CONSENSUS SEQUENCES J. Biol. Chem., August 17, 2001; 276(34): 31642 - 31650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gonzalez-Reyes, M. B. Ruiz-Arguello, B. Garcia-Barreno, L. Calder, J. A. Lopez, J. P. Albar, J. J. Skehel, D. C. Wiley, and J. A. Melero Cleavage of the human respiratory syncytial virus fusion protein at two distinct sites is required for activation of membrane fusion PNAS, August 14, 2001; 98(17): 9859 - 9864. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
